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dc.contributor.authorMelinek, Beatrice J
dc.contributor.authorDessoy, Sandrine
dc.contributor.authorWright, Bernice
dc.contributor.authorBracewell, Dan G
dc.contributor.authorMukhopadhyay, Tarit K
dc.date.accessioned2019-08-13T10:04:01Z
dc.date.available2019-08-13T10:04:01Z
dc.date.issued2018-01-08
dc.identifier.citationMelinek B. J., Dessoy S., Wright B., Bracewell, D. G. and Mukhopadhyay, T. K. (2018) Ultra scale-down approaches to study the centrifugal harvest for viral vaccineproduction, Biotechnology and Bioengineering, 115(5), pp. 1226–1238.en
dc.identifier.issn0006-3592en
dc.identifier.doi10.1002/bit.26546en
dc.identifier.urihttp://hdl.handle.net/2436/622644
dc.description.abstractLarge scale continuous cell‐line cultures promise greater reproducibility and efficacy for the production of influenza vaccines, and adenovirus for gene therapy. This paper seeks to use an existing validated ultra scale‐down tool, which is designed to mimic the commercial scale process environment using only milliliters of material, to provide some initial insight into the performance of the harvest step for these processes. The performance of industrial scale centrifugation and subsequent downstream process units is significantly affected by shear. The properties of these cells, in particular their shear sensitivity, may be changed considerably by production of a viral product, but literature on this is limited to date. In addition, the scale‐down tool used here has not previously been applied to the clarification of virus production processes. The results indicate that virus infected cells do not actually show any increase in sensitivity to shear, and may indeed become less shear sensitive, in a similar manner to that previously observed in old or dead cell cultures. Clarification may be most significantly dependent on the virus release mechanism, with the budding influenza virus producing a much greater decrease in clarification than the lytic, non‐enveloped adenovirus. A good match was also demonstrated to the industrial scale performance in terms of clarification, protein release, and impurity profile.en
dc.description.sponsorshipUK Engineering and Physical Sciences Research Council, EPSRC. Grant Number: EP/G034656/1en
dc.formatapplication/PDFen
dc.languageen
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/full/10.1002/bit.26546en
dc.subjectcentrifugationen
dc.subjectgene-therapyen
dc.subjectscale-downen
dc.subjectshearen
dc.subjectviral vaccineen
dc.titleUltra scale-down approaches to study the centrifugal harvest for viral vaccine productionen
dc.typeJournal articleen
dc.identifier.journalBiotechnology and Bioengineeringen
dc.date.updated2019-08-12T13:16:32Z
dc.date.accepted2018-01-03
rioxxterms.funderEPSRCen
rioxxterms.identifier.projectEP/G034656/1en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2019-08-13en
dc.source.volume115
dc.source.issue5
dc.source.beginpage1226
dc.source.endpage1238
dc.description.versionPublished version
refterms.dateFCD2019-08-13T10:02:56Z
refterms.versionFCDAM
refterms.dateFOA2019-08-13T10:04:02Z


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