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dc.contributor.authorMcRonald, FE
dc.contributor.authorMorris, MR
dc.contributor.authorGentle, D
dc.contributor.authorWinchester, L
dc.contributor.authorBaban, D
dc.contributor.authorRagoussis, J
dc.contributor.authorClarke, NW
dc.contributor.authorBrown, MD
dc.contributor.authorKishida, T
dc.contributor.authorYao, M
dc.contributor.authorLatif, F
dc.contributor.authorMaher, ER
dc.date.accessioned2019-07-11T14:47:13Z
dc.date.available2019-07-11T14:47:13Z
dc.date.issued2009-06-03
dc.identifier.citationMcRonald, F. E., Morris, M. R., Gentle, D., Winchester, L., Baban, D., Ragoussis, J., Clarke, N. W., Brown, M. D., Kishida, T., Yao, M., Latif, F. and Maher, E. R. (2009) CpG methylation profiling in VHL related and VHL unrelated renal cell carcinoma, Molecular Cancer (2009), 8, 31.en
dc.identifier.pmid19493342
dc.identifier.doi10.1186/1476-4598-8-31en
dc.identifier.urihttp://hdl.handle.net/2436/622542
dc.description.abstractBackground: Renal cell carcinoma (RCC) is histopathologically heterogeneous with clear cell and papillary the most common subtypes. The most frequent molecular abnormality in clear cell RCC is VHL inactivation but promoter methylation of tumour suppressor genes is common in both subtypes of RCC. To investigate whether RCC CpG methylation status was influenced by histopathology and VHL status we performed high-throughput epigenetic profiling using the Illumina Goldengate Methylation Array in 62 RCC (29 RCC from von Hippel-Lindau (VHL) disease patients, 20 sporadic clear cell RCC with wild type VHL and 13 sporadic papillary RCC). Results: 43 genes were methylated in >20% of primary RCC (range 20-45%) and most (37/43) of these had not been reported previously to be methylated in RCC. The distribution of the number of methylated CpGs in individual tumours differed from the expected Poisson distribution (p < 0.00001; log-likelihood G test) suggesting that a subset of RCC displayed a CpG Island Methylator Phenotype. Comparison of RCC subtypes revealed that, on average, tumour specific CpG methylation was most prevalent in papillary RCC and least in VHL RCC. Many of the genes preferentially methylated in pRCC were linked to TGFβ or ERK/Akt signalling. Conclusion: These findings demonstrate differing patterns of tumour-specific CpG methylation in VHL and non VHL clear cell RCC and papillary RCC, and identify multiple novel potential CpG methylation biomarkers for RCC. © 2009 McRonald et al; licensee BioMed Central Ltd.en
dc.description.sponsorshipCancer Research UKen
dc.formatapplication/PDFen
dc.languageeng
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urlhttps://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-8-31en
dc.rightsLicence for published version: Creative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHumansen
dc.subjectCarcinoma, Renal Cellen
dc.subjectKidney Neoplasmsen
dc.subjectCluster Analysisen
dc.subjectPoisson Distributionen
dc.subjectStatistics, Nonparametricen
dc.subjectReproducibility of Resultsen
dc.subjectDNA Methylationen
dc.subjectEpigenesis, Geneticen
dc.subjectCpG Islandsen
dc.subjectAdulten
dc.subjectAgeden
dc.subjectMiddle Ageden
dc.subjectGene Regulatory Networksen
dc.subjectvon Hippel-Lindau Diseaseen
dc.titleCpG methylation profiling in VHL related and VHL unrelated renal cell carcinomaen
dc.typeJournal articleen
dc.identifier.eissn1476-4598
dc.identifier.journalMolecular Canceren
dc.date.updated2019-06-24T15:35:15Z
dc.contributor.institutionCancer Research UK Renal Molecular Oncology Group, University of Birmingham, Birmingham, UK. f.e.mcronald@bham.ac.uk
pubs.place-of-publicationEngland
dc.date.accepted2009-06-03
rioxxterms.funderEMBO and ESFen
rioxxterms.identifier.projectG0500966en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2019-07-11en
dc.source.volume8
dc.source.issue1
dc.source.beginpage31
dc.description.versionPublished version
refterms.dateFCD2019-07-11T14:47:04Z
refterms.versionFCDVoR
refterms.dateFOA2019-07-11T14:47:13Z


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Licence for published version: Creative Commons Attribution 4.0 International
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