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dc.contributor.authorClark, Alice
dc.contributor.authorSavva, CG
dc.contributor.authorNaylor, CE
dc.contributor.authorPopoff, MR
dc.contributor.authorMoss, DS
dc.contributor.authorBasak, AK
dc.contributor.authorTitball, RW
dc.contributor.authorBokori-Brown, M
dc.date.accessioned2019-07-10T14:05:53Z
dc.date.available2019-07-10T14:05:53Z
dc.date.issued2019-06-14
dc.identifier.citationSavva, G. et al The pore structure of Clostridium perfringensepsilon toxin, Nature Communications, 10, DOI: 10.1038/s41467-019-10645-8en
dc.identifier.issn2041-1723en
dc.identifier.doi10.1038/s41467-019-10645-8en
dc.identifier.urihttp://hdl.handle.net/2436/622535
dc.description.abstractEpsilon toxin (Etx), a potent pore forming toxin (PFT) produced by Clostridium perfringens, is responsible for the pathogenesis of enterotoxaemia of ruminants and has been suggested to play a role in multiple sclerosis in humans. Etx is a member of the aerolysin family of β-PFTs (aβ-PFTs). While the Etx soluble monomer structure was solved in 2004, Etx pore structure has remained elusive due to the difficulty of isolating the pore complex. Here we show the cryo-electron microscopy structure of Etx pore assembled on the membrane of susceptible cells. The pore structure explains important mutant phenotypes and suggests that the double β-barrel, a common feature of the aβ-PFTs, may be an important structural element in driving efficient pore formation. These insights provide the framework for the development of novel therapeutics to prevent human and animal infections, and are relevant for nano-biotechnology applications.en
dc.formatapplication/pdfen
dc.languageEnglish
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urlhttps://www.nature.com/articles/s41467-019-10645-8en
dc.subjectporeen
dc.subjectproteinen
dc.titleThe pore structure of Clostridium perfringensepsilon toxinen
dc.typeJournal articleen
dc.identifier.journalNature Communicationsen
dc.date.updated2019-07-09T14:09:37Z
dc.date.accepted2019-05-20
rioxxterms.funderUniversity of Wolverhamptonen
rioxxterms.identifier.projectMC-A021-53019en
rioxxterms.identifier.projectWT089618MAen
rioxxterms.versionAMen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2019-07-10en
refterms.dateFCD2019-07-10T14:05:35Z
refterms.versionFCDAM
refterms.dateFOA2019-07-10T14:05:53Z


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