Authors
Savva, Christos G.Clark, Alice
Naylor, Claire E.
Popoff, Michel R.
Moss, David S.
Basak, Ajit K.
Titball, Richard W.
Bokori-Brown, Monika
Issue Date
2019-06-14
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Show full item recordAbstract
Epsilon toxin (Etx), a potent pore forming toxin (PFT) produced by Clostridium perfringens, is responsible for the pathogenesis of enterotoxaemia of ruminants and has been suggested to play a role in multiple sclerosis in humans. Etx is a member of the aerolysin family of β-PFTs (aβ-PFTs). While the Etx soluble monomer structure was solved in 2004, Etx pore structure has remained elusive due to the difficulty of isolating the pore complex. Here we show the cryo-electron microscopy structure of Etx pore assembled on the membrane of susceptible cells. The pore structure explains important mutant phenotypes and suggests that the double β-barrel, a common feature of the aβ-PFTs, may be an important structural element in driving efficient pore formation. These insights provide the framework for the development of novel therapeutics to prevent human and animal infections, and are relevant for nano-biotechnology applications.Citation
Savva, C.G., Clark, A.R., Naylor, C.E. et al. (2019) The pore structure of Clostridium perfringens epsilon toxin. Nature Communications, 10, 2641. https://doi.org/10.1038/s41467-019-10645-8Publisher
Springer NatureJournal
Nature CommunicationsAdditional Links
https://www.nature.com/articles/s41467-019-10645-8Type
Journal articleLanguage
enDescription
© 2019 The Authors. Published by Springer. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1038/s41467-019-10645-8ISSN
2041-1723ae974a485f413a2113503eed53cd6c53
10.1038/s41467-019-10645-8
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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/