Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
Abstract
Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of GTP in this process. The part of light scattering theory that is essential for kinetic measurements is high-lighted in the main text and a more general treatment of Rayleigh scattering from macromolecules is given in an appendix.Citation
Antoun, A., Pavlov, M. Y., Tenson, T. and Ehrenberg, M. (2004) Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering, Biological Procedures Online, 6(1), pp. 35-54.Publisher
Springer NatureJournal
Biological Procedures OnlineDOI
10.1251/bpo71PubMed ID
15103398Type
Journal articleLanguage
enEISSN
1480-9222ae974a485f413a2113503eed53cd6c53
10.1251/bpo71
Scopus Count
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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/
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