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dc.contributor.authorJones, Sarah
dc.contributor.authorOsman, Shaimaa
dc.contributor.authorHowl, John
dc.date.accessioned2019-04-12T12:38:33Z
dc.date.available2019-04-12T12:38:33Z
dc.date.issued2019-02-20
dc.identifier.citationJones S, Osman S, Howl J. The planarian Schmidtea mediterranea as a model system for the discovery and characterization of cell- penetrating peptides and bioportides. Chemical Biology & Drug Design, 2019, pp. 1–14.en
dc.identifier.issn1747-0277en
dc.identifier.doi10.1111/cbdd.13483en
dc.identifier.urihttp://hdl.handle.net/2436/622281
dc.description.abstractThe general utility of the planarian Schmidtea mediterranea, an organism with remarkable regenerative capacity, was investigated as a convenient three‐dimensional model to analyse the import of cell‐penetrating peptides (CPPs) and bioportides (bioactive CPPs) into complex tissues. The unpigmented planarian blastema, 3 days post head amputation, is a robust platform to assess the penetration of red‐fluorescent CPPs into epithelial cells and deeper tissues. Three planarian proteins, Ovo, ZicA and Djeya, which collectively control head remodelling and eye regeneration following decapitation, are a convenient source of novel cationic CPP vectors. One example, Djeya1 (RKLAFRYRRIKELYNSYR), is a particularly efficient and seemingly inert CPP vector that could be further developed to assist the delivery of bioactive payloads across the plasma membrane of eukaryotic cells. Eye regeneration, following head amputation, was utilized in an effort to identify bioportides capable of influencing stem cell‐dependent morphogenesis. These investigations identified the tetradecapeptide mastoparan (INLKALAALAKKIL) as a bioportide able to influence the gross morphology of head development. We conclude that, compared with cellular monolayers, the S. mediterranea system provides many advantages and will support the identification of bioportides able to selectively modify the biology of totipotent neoblasts and, presumably, other mammalian stem cell types.en
dc.formatapplication/PDFen
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/cbdd.13483en
dc.rightsAttribution-NonCommercial 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/us/*
dc.subjectbioportideen
dc.subjectblastemaen
dc.subjectcell-penetrating peptideen
dc.subjectconfocal microscopyen
dc.subjecteye regenerationen
dc.subjectpeptide synthesisen
dc.titleThe planarian Schmidtea mediterranea as a model system for the discovery and characterization of cell penetrating peptides and bioportidesen
dc.typeJournal articleen
dc.identifier.journalChemical Biology & Drug Designen
dc.date.accepted2018-12-27
rioxxterms.funderUniversity of Wolverhampton and Newton-Mosharafa Fellowshipen
rioxxterms.identifier.projectUOW120419SJen
rioxxterms.versionAMen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc/4.0/en
rioxxterms.licenseref.startdate2020-02-20en
dc.source.volume2019
dc.source.beginpage1
dc.source.endpage14
refterms.dateFCD2019-04-12T12:38:10Z
refterms.versionFCDAM


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