Show simple item record

dc.contributor.authorSavage, Aaron M
dc.contributor.authorKurusamy, Sathishkumar
dc.contributor.authorChen, Yan
dc.contributor.authorJiang, Zhen
dc.contributor.authorChhabria, Karishma
dc.contributor.authorMacDonald, Ryan B.
dc.contributor.authorKim, Hyejeong R.
dc.contributor.authorWilson, Heather L.
dc.contributor.authorvan Eeden, Fredericus J.M.
dc.contributor.authorArmesilla, Angel L.
dc.contributor.authorChico, Timothy J.A.
dc.contributor.authorWilkinson, Robert N.
dc.date.accessioned2019-02-20T14:20:24Z
dc.date.available2019-02-20T14:20:24Z
dc.date.issued2019-02-13
dc.identifier.citationSavage, AM., Kurusamy, S., Chen, Y., Jiang, Z., Chhabria, K., MacDonald, RB., Kim, HR., Wilson, HL., van Eeden, FJM., Armesilla, AL., Chico, TJA., Wilkinson, RN. (2019) 'tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis', Nature Communications, 10(1) p. 732. doi: 10.1038/s41467-019-08590-7
dc.identifier.issn2041-1723
dc.identifier.doi10.1038/s41467-019-08590-7
dc.identifier.urihttp://hdl.handle.net/2436/622103
dc.description.abstractAngiogenesis requires co-ordination of multiple signalling inputs to regulate the behaviour of endothelial cells (ECs) as they form vascular networks. Vascular endothelial growth factor (VEGF) is essential for angiogenesis and induces downstream signalling pathways including increased cytosolic calcium levels. Here we show that transmembrane protein 33 (tmem33), which has no known function in multicellular organisms, is essential to mediate effects of VEGF in both zebrafish and human ECs. We find that tmem33 localises to the endoplasmic reticulum in zebrafish ECs and is required for cytosolic calcium oscillations in response to Vegfa. tmem33-mediated endothelial calcium oscillations are critical for formation of endothelial tip cell filopodia and EC migration. Global or endothelial-cell-specific knockdown of tmem33 impairs multiple downstream effects of VEGF including ERK phosphorylation, Notch signalling and embryonic vascular development. These studies reveal a hitherto unsuspected role for tmem33 and calcium oscillations in the regulation of vascular development.
dc.formatapplication/PDF
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.urlhttps://www.nature.com/articles/s41467-019-08590-7
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.subjecttmem33
dc.subjectangiogenesis
dc.subjectVEGF
dc.subjectcalcium
dc.titletmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis
dc.typeJournal article
dc.identifier.journalNature Communications
dc.identifier.articlenumber732
dc.date.accepted2019-01-21
rioxxterms.funderUniversity of Wolverhampton
rioxxterms.identifier.projectUOW200219AA
rioxxterms.versionAM
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
rioxxterms.licenseref.startdate2019-02-20
dc.source.volume10
refterms.dateFCD2019-02-20T14:20:25Z
refterms.versionFCDAM
refterms.dateFOA2019-02-20T14:20:25Z


Files in this item

Thumbnail
Name:
Savage_et_al_tmem33_2019.pdf
Size:
5.166Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States