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dc.contributor.authorMitrou, Georgia I.
dc.contributor.authorSakkas, Georgos K.
dc.contributor.authorPoulianiti, Konstantina P.
dc.contributor.authorKarioti, Aggeliki
dc.contributor.authorTepetes, Konstantinos
dc.contributor.authorChristodoulidis, Grigorios
dc.contributor.authorGiakas, Giannis
dc.contributor.authorStefanidis, Ioannis
dc.contributor.authorGeeves, Michael A.
dc.contributor.authorKoutedakis, Yiannis
dc.contributor.authorKaratzaferi, Christina
dc.date.accessioned2019-01-16T14:50:32Z
dc.date.available2019-01-16T14:50:32Z
dc.date.issued2018-11-03
dc.identifier.citationMitrou, G.I. et al (2018) Evidence of functional deficits at the single muscle fiber level in experimentally-induced renal insufficiency, Journal of Biomechanics. doi 10.1016/j.jbiomech.2018.10.035en
dc.identifier.issn0021-9290en
dc.identifier.doi10.1016/j.jbiomech.2018.10.035
dc.identifier.urihttp://hdl.handle.net/2436/622057
dc.description.abstractChronic kidney disease patients present with metabolic and functional muscle abnormalities, called uremic myopathy, whose mechanisms have not yet been fully elucidated. We investigated whether chronic renal insufficiency (CRI) affects skeletal muscle contractile properties at the cellular level. CRI was induced surgically in New Zealand rabbits (UREM), with sham-operation for controls (CON), and samples were collected at 3 months post-surgery, following euthanasia. All protocols had University Ethics approval following national and European guidelines. Sample treatments and evaluations were blinded. Maximal isometric force was assessed in 382 permeabilized psoas fibers (CON, n = 142, UREM, n = 240) initially at pH7, 10 °C (‘standard’ conditions), in subsets of fibers in acidic conditions (pH6.2, 10 °C) but also at near physiological temperature (pH7, 30 °C and pH6.2, 30 °C). CRI resulted in significant smaller average cross sectional areas (CSAs) by ∼11% for UREM muscle fibers (vs CON, P < 0.01). At standard conditions, UREM fibers produced lower absolute and specific forces (i.e. normalized force per fiber CSA) (vs CON, P < 0.01); force increased in 30 °C for both groups (P < 0.01), but the disparity between UREM and CON remained significant. Acidosis significantly reduced force (vs pH7, 10 °C P < 0.01), similarly in both groups (in UREM by −48% and in CON by −43%, P > 0.05). For the first time, we give evidence that CRI can induce significant impairments in single psoas muscle fibers force generation, only partly explained by fiber atrophy, thus affecting muscle mechanics at the cellular level.en
dc.formatapplication/PDFen
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0021929018308248?via%3Dihuben
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectchronic kidney diseaseen
dc.subjectisometric tensionen
dc.subjectskinned fibersen
dc.subjectspecific forceen
dc.subjectanimal modelen
dc.subjecturemic myopathyen
dc.titleEvidence of functional deficits at the single muscle fiber level in experimentally-induced renal insufficiencyen
dc.typeJournal article
dc.identifier.journalJournal of Biomechanicsen
dc.date.accepted2018-10-30
rioxxterms.funderUniversity of Wolverhamptonen
rioxxterms.identifier.projectUOW160119YKen
rioxxterms.versionAMen
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2019-11-03en
dc.source.volume82
dc.source.issueJanuary
dc.source.beginpage259
dc.source.endpage265
refterms.dateFCD2019-01-16T14:50:33Z
refterms.versionFCDAM


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