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dc.contributor.authorRajasekar, Adharsh
dc.contributor.authorWilkinson, Stephen
dc.contributor.authorSekar, Raju
dc.contributor.authorBridge, Jonathan
dc.contributor.authorMedina-Roldán, Eduardo
dc.contributor.authorK.S. Moy, Charles
dc.date.accessioned2018-11-16T15:39:24Z
dc.date.available2018-11-16T15:39:24Z
dc.date.issued2018-08-27
dc.identifier.citationRajasekar, A., Wilkinson, S., Sekar, R., Bridge,, J., Medina-Roldán, E., Moy, CKS. (2018) 'Biomineralisation performance of bacteria isolated from a landfill in China', Canadian Journal of Microbiology, 1 (9) DOI: 10.1139/cjm-2018-0254
dc.identifier.issn0008-4166
dc.identifier.doi10.1139/cjm-2018-0254
dc.identifier.urihttp://hdl.handle.net/2436/621896
dc.description.abstractWe report an investigation of microbially induced carbonate precipitation by seven indigenous bacteria isolated from a landfill in China. Bacterial strains were cultured in a medium supplemented with 25 mmol/L calcium chloride and 333 mmol/L urea. The experiments were carried out at 30 °C for 7 days with agitation by a shaking table at 130 r/min. Scanning electron microscopic and X-ray diffraction analyses showed variations in calcium carbonate polymorphs and mineral composition induced by all bacterial strains. The amount of carbonate precipitation was quantified by titration. The amount of carbonate precipitated in the medium varied among isolates, with the lowest being Bacillus aerius rawirorabr15 (LC092833) precipitating around 1.5 times more carbonate per unit volume than the abiotic (blank) solution. Pseudomonas nitroreducens szh_asesj15 (LC090854) was found to be the most efficient, precipitating 3.2 times more carbonate than the abiotic solution. Our results indicate that bacterial carbonate precipitation occurred through ureolysis and suggest that variations in carbonate crystal polymorphs and rates of precipitation were driven by strain-specific differences in urease expression and response to the alkaline environment. These results and the method applied provide benchmarking and screening data for assessing the bioremediation potential of indigenous bacteria for containment of contaminants in landfills.
dc.description.sponsorshipXJTLU Research Development Fund
dc.formatapplication/PDF
dc.language.isoen
dc.relation.urlhttps://www.nrcresearchpress.com/doi/10.1139/cjm-2018-0254
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.subjectbiomineralisation
dc.subjectindigenous bacteria
dc.subjectlandfill
dc.subjectBacillus
dc.subjectPseudomonas
dc.subjectSEM
dc.titleBiomineralisation performance of bacteria isolated from a landfill in China
dc.typeJournal article
dc.identifier.journalCanadian Journal of Microbiology
dc.date.accepted2018-08-07
rioxxterms.funderUniversity of Wolverhampton
rioxxterms.identifier.projectUOW16112018SW
rioxxterms.versionAM
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
rioxxterms.licenseref.startdate2018-08-27
dc.source.volume64
dc.source.issue12
dc.source.beginpage945
dc.source.endpage953
refterms.dateFCD2018-11-16T15:39:25Z
refterms.versionFCDAM
refterms.dateFOA2018-11-16T15:39:25Z


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