Poly-Gamma-Glutamic Acid (γ-PGA)-based encapsulation of Adenovirus to evade neutralizing antibodies.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsKhalil, Ibrahim R
Khechara, Martin P
Armesilla, Angel L
Focarete, Maria Letizia
MetadataShow full item record
AbstractIn recent years, there has been an increasing interest in oncolytic adenoviral vectors as an alternative anticancer therapy. The induction of an immune response can be considered as a major limitation of this kind of application. Significant research efforts have been focused on the development of biodegradable polymer poly-gamma-glutamic acid (γ-PGA)-based nanoparticles used as a vector for effective and safe anticancer therapy, owing to their controlled and sustained-release properties, low toxicity, as well as biocompatibility with tissue and cells. This study aimed to introduce a specific destructive and antibody blind polymer-coated viral vector into cancer cells using γ-PGA and chitosan (CH). Adenovirus was successfully encapsulated into the biopolymer particles with an encapsulation efficiency of 92% and particle size of 485 nm using the ionic gelation method. Therapeutic agents or nanoparticles (NPs) that carry therapeutics can be directed specifically to cancerous cells by decorating their surfaces using targeting ligands. Moreover, in vitro neutralizing antibody response against viral capsid proteins can be somewhat reduced by encapsulating adenovirus into γ-PGA-CH NPs, as only 3.1% of the encapsulated adenovirus was detected by anti-adenovirus antibodies in the presented work compared to naked adenoviruses. The results obtained and the unique characteristics of the polymer established in this research could provide a reference for the coating and controlled release of viral vectors used in anticancer therapy.
SponsorsThis work was funded by the Ministry of Higher Education and Scientific Research (Iraq). This work was also partially funded by the Research Investment Fund, University of Wolverhampton (Wolverhampton, United Kingdom) and the Italian Ministry of University and Research (MIUR).
- Pro-inflammatory chitosan/poly(γ-glutamic acid) nanoparticles modulate human antigen-presenting cells phenotype and revert their pro-invasive capacity.
- Authors: Castro F, Pinto ML, Silva AM, Pereira CL, Teixeira GQ, Gomez-Lazaro M, Santos SG, Barbosa MA, Gonçalves RM, Oliveira MJ
- Issue date: 2017 Nov
- Bacterial-Derived Polymer Poly-y-Glutamic Acid (y-PGA)-Based Micro/Nanoparticles as a Delivery System for Antimicrobials and Other Biomedical Applications.
- Authors: Khalil IR, Burns AT, Radecka I, Kowalczuk M, Khalaf T, Adamus G, Johnston B, Khechara MP
- Issue date: 2017 Feb 2
- Chitosan cross-linked docetaxel loaded EGF receptor targeted nanoparticles for lung cancer cells.
- Authors: Maya S, Sarmento B, Lakshmanan VK, Menon D, Seabra V, Jayakumar R
- Issue date: 2014 Aug
- [Efficacy and safety of poly (gamma-glutamic acid) based nanoparticles (gamma-PGA NPs) as vaccine carrier].
- Authors: Nakagawa S
- Issue date: 2008 Nov
- Biodegradable poly(D,L-lactide-co-glycolide)/poly(L-γ-glutamic acid) nanoparticles conjugated to folic acid for targeted delivery of doxorubicin.
- Authors: Jaimes-Aguirre L, Morales-Avila E, Ocampo-García BE, Medina LA, López-Téllez G, Gibbens-Bandala BV, Izquierdo-Sánchez V
- Issue date: 2017 Jul 1