Assessment of the Pro12Ala polymorphism in the PPAR-γ2 gene among type 2 diabetes patients in a Nigerian population
Authors
Engwa, Godwill AzehNwalo, Friday Nweke
Chiezey, Venatus Osita
Unachukwu, Marian N
Ojo, Opeolu Oyejide
Ubi, Benjamin Ewa
Issue Date
2018-04-05
Metadata
Show full item recordAbstract
The association between the Pro12Ala polymorphism of the PPARγ2 gene, type 2 diabetes (T2D), and obesity in certain ethnic populations has been reported. However, this relationship has not yet been described among diabetes patients in Nigeria. This study investigated the relationship between the Pro12Ala polymorphism in the PPARγ2 gene, obesity, and lipid abnormalities characterizing T2D among patients in Nigeria. This case-control study recruited 73 T2D and 75 non-diabetic (ND) patients. Demographic and clinical data were collected and blood glucose levels together with serum lipid profile for patients were measured. Pro12Ala polymorphism in the PPARγ2 gene was genotyped by restriction fragment length-Polymerase Chain Reaction (RFLP-PCR). The PPAR-γ2 gene (amplicon size = 270 base pair) was successfully amplified for all samples. Following restriction enzyme digestion and analysis by agarose gel electrophoresis, amplicons from samples showed a band of size 270 bp and were of the wild homozygous Pro/Pro genotype. Ala12 variant was totally absent from the study population. Obesity, estimated using Body Mass Index (BMI) and waist circumference (WC), was significantly higher (p < 0.05) in T2D patients compared to the non-diabetic patients. More so, the prevalence of lipid abnormalities; hypercholesterolaemia (TC > 200 mg/dL), hypertriglyceridaemia (TG > 150 mg/dL), high HDL (>100 mg/dL), and low HDL (<50 mg/dL) was significantly greater (p < 0.001) in T2D patients compared to non-diabetic patients. Results obtained further indicated lack of significant association between PPAR-γ2 gene polymorphism, T2D, and obesity. However, obesity and dyslipidaemia were strongly associated in T2D patients.Citation
Engwa GA., Nwalo FN., Chiezey VO., Unachukwu MN., Ojo OO., Ubi BE. 'Assessment of the Pro12Ala Polymorphism in the PPAR-γ2 Gene among Type 2 Diabetes Patients in a Nigerian Population', Journal of Clinical Medicine, 7 (4) p. 69Publisher
MDPIJournal
Journal of Clinical MedicinePubMed ID
29621178Additional Links
http://www.mdpi.com/2077-0383/7/4/69Type
Journal articleLanguage
enISSN
2077-0383ae974a485f413a2113503eed53cd6c53
10.3390/jcm7040069
Scopus Count
Collections
The following licence applies to the copyright and re-use of this item:
- Creative Commons
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/
Related articles
- The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population.
- Authors: Mato EPM, Pokam-Fosso PE, Atogho-Tiedeu B, Noubiap JJN, Evehe MS, Djokam-Dadjeu R, Donfack OS, Ngwa EN, Guewo-Fokeng M, Mbacham WF, Sobngwi E, Mbanya JC
- Issue date: 2016
- Association between Pro12Ala polymorphism of the PPAR-gamma2 gene and insulin sensitivity in Brazilian patients with type-2 diabetes mellitus.
- Authors: Tavares V, Hirata RD, Rodrigues AC, Monte O, Salles JE, Scalissi N, Speranza AC, Hirata MH
- Issue date: 2005 Sep
- PPAR-gamma2 pro12Ala polymorphism is associated with post-challenge abnormalities of glucose homeostasis in children and adolescents with obesity.
- Authors: Jermendy A, Körner A, Kovács M, Madácsy L, Cseh K
- Issue date: 2011
- Association of Pro12Ala polymorphism in the peroxisome proliferator-activated receptor gamma2 gene with small dense low-density lipoprotein in the general population.
- Authors: Hamada T, Kotani K, Tsuzaki K, Sano Y, Murata T, Tabata M, Sato S, Sakane N
- Issue date: 2007 Oct
- Association of the PRO12ALA polymorphism of the PPAR-gamma2 gene with oxidized low-density lipoprotein and cardiolipin autoantibodies in nondiabetic and type 2 diabetic subjects.
- Authors: Niskanen L, Lindi V, Erkkilä A, Sivenius K, Luoma J, Ylä-Herttuala S, Laakso M, Uusitupa MI
- Issue date: 2003 Feb