Assessment of the Pro12Ala polymorphism in the PPAR-γ2 gene among type 2 diabetes patients in a Nigerian population
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsEngwa, Godwill Azeh
Nwalo, Friday Nweke
Chiezey, Venatus Osita
Unachukwu, Marian N
Ojo, Opeolu Oyejide
Ubi, Benjamin Ewa
MetadataShow full item record
AbstractThe association between the Pro12Ala polymorphism of the PPARγ2 gene, type 2 diabetes (T2D), and obesity in certain ethnic populations has been reported. However, this relationship has not yet been described among diabetes patients in Nigeria. This study investigated the relationship between the Pro12Ala polymorphism in the PPARγ2 gene, obesity, and lipid abnormalities characterizing T2D among patients in Nigeria. This case-control study recruited 73 T2D and 75 non-diabetic (ND) patients. Demographic and clinical data were collected and blood glucose levels together with serum lipid profile for patients were measured. Pro12Ala polymorphism in the PPARγ2 gene was genotyped by restriction fragment length-Polymerase Chain Reaction (RFLP-PCR). The PPAR-γ2 gene (amplicon size = 270 base pair) was successfully amplified for all samples. Following restriction enzyme digestion and analysis by agarose gel electrophoresis, amplicons from samples showed a band of size 270 bp and were of the wild homozygous Pro/Pro genotype. Ala12 variant was totally absent from the study population. Obesity, estimated using Body Mass Index (BMI) and waist circumference (WC), was significantly higher (p < 0.05) in T2D patients compared to the non-diabetic patients. More so, the prevalence of lipid abnormalities; hypercholesterolaemia (TC > 200 mg/dL), hypertriglyceridaemia (TG > 150 mg/dL), high HDL (>100 mg/dL), and low HDL (<50 mg/dL) was significantly greater (p < 0.001) in T2D patients compared to non-diabetic patients. Results obtained further indicated lack of significant association between PPAR-γ2 gene polymorphism, T2D, and obesity. However, obesity and dyslipidaemia were strongly associated in T2D patients.
CitationEngwa GA., Nwalo FN., Chiezey VO., Unachukwu MN., Ojo OO., Ubi BE. 'Assessment of the Pro12Ala Polymorphism in the PPAR-γ2 Gene among Type 2 Diabetes Patients in a Nigerian Population', Journal of Clinical Medicine, 7 (4) p. 69
JournalJournal of Clinical Medicine
The following license files are associated with this item:
- The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population.
- Authors: Mato EPM, Pokam-Fosso PE, Atogho-Tiedeu B, Noubiap JJN, Evehe MS, Djokam-Dadjeu R, Donfack OS, Ngwa EN, Guewo-Fokeng M, Mbacham WF, Sobngwi E, Mbanya JC
- Issue date: 2016
- Association between Pro12Ala polymorphism of the PPAR-gamma2 gene and insulin sensitivity in Brazilian patients with type-2 diabetes mellitus.
- Authors: Tavares V, Hirata RD, Rodrigues AC, Monte O, Salles JE, Scalissi N, Speranza AC, Hirata MH
- Issue date: 2005 Sep
- A possible association of Pro12Ala polymorphism in peroxisome proliferator-activated receptor gamma2 gene with obesity in native Javanese in Indonesia.
- Authors: Danawati CW, Nagata M, Moriyama H, Hara K, Yasuda H, Nakayama M, Kotani R, Yamada K, Sakata M, Kurohara M, Wiyono P, Asdie H, Sakaue M, Taniguchi H, Yokono K
- Issue date: 2005 Sep-Oct
- Possible association between ABCC8 C49620T polymorphism and type 2 diabetes in a Nigerian population.
- Authors: Engwa GA, Nwalo FN, Chikezie CC, Onyia CO, Ojo OO, Mbacham WF, Ubi BE
- Issue date: 2018 May 12
- [Association of the Pro12Ala and C1431T polymorphism of the PPAR gamma2 gene and their haplotypes with obesity and type 2 diabetes].
- Authors: Dong CP, He L, Li JN, Ye F, He M, Wang Y
- Issue date: 2008 Aug