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dc.contributor.authorZhang, Wei
dc.contributor.authorLiu, Xiaoqin
dc.contributor.authorFan, Huajun
dc.contributor.authorZhu, Dan
dc.contributor.authorWu, Xuehao
dc.contributor.authorHuang, Xiaowen
dc.contributor.authorTang, James
dc.date.accessioned2018-07-26T14:56:33Z
dc.date.available2018-07-26T14:56:33Z
dc.date.issued2016-04-06
dc.identifier.issn09266690
dc.identifier.doi10.1016/j.indcrop.2016.03.052
dc.identifier.urihttp://hdl.handle.net/2436/621533
dc.description.abstractBased on advantages of microwave-assisted extraction (MAE), aqueous two-phase extraction (ATPE)and reversed micellar extraction (RME), a novel method of extraction and purification of alkaloids fromSophora flavescens Ait. was put forward by using focused microwave-assisted aqueous two-phase extrac-tion (FMAATPE) coupled with the reversed micellar extraction (RME). The aqueous two-phase system(ATPS) consisted of ethanol and ammonia sulfate was used as extracting solvent, FMAATPE conditionssuch as the compositions of ATPS, and the extraction temperature, extraction time and solvent-to-materials ratio were optimized by orthogonal test through HPLC analysis. Then alkaloids extracted byFMAATPE were further purified with reverse micelles of sodium dodecyl benzene sulfonate/isooctane/n-octanol. The relative purity of total alkaloids in the product was improved greatly by RME from 58.30% to88.75% (w/w), and purification recovery was higher than that of solvent extraction, ion exchange resinand macroporous adsorption resin. Moreover, the reverse micelles used could be readily recycled byadding salt and water, keeping better extraction performance with about 80% (w/w) of recovery. Thus,the proposed method is easy to adjust, operate and enlarge for better application prospect; the purity ofalkaloids obtained meets the requirements of the compound drug preparation.
dc.description.sponsorshipThe Science and Technology Project of Guangzhou (No. 2008Z1-E301) and Faculty Development fund Project of Guangdong Pharmaceutical University (No. 52104109)
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0926669016302126
dc.subjectFocused microwave-assisted aqueous two-phase extraction
dc.subjectReversed micellar extraction
dc.subjectSophora flavescens Ait.
dc.subjectAlkaloids
dc.subjectHigh performance liquid chromatography
dc.titleSeparation and purification of alkaloids from Sophora flavescens Ait. by focused microwave-assisted aqueous two-phase extraction coupled with reversed micellar extraction
dc.typeJournal article
dc.identifier.journalIndustrial Crops and Products
dc.date.accepted2016-03-30
dc.source.journaltitleIndustrial Crops and Products
dc.source.volume86
dc.source.beginpage231
dc.source.endpage238


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