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    Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model

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    Authors
    Dong, Cui-Ling
    Webb, William R.
    Peng, Qiang
    Tang, James Z.
    Forsyth, Nicholas R.
    Chen, Guo-Qiang
    El Haj, Alicia J.
    Issue Date
    2014-02
    
    Metadata
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    Abstract
    This study aimed to design a growth factor loaded copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) nanoparticles containing 3D collagen matrix to achieve growth factor sustained release for long-term stimulation of human mesenchymal stem cells (hMSCs) proliferation/differentiation for tissue engineer application. Platelet-derived growth factor-BB (PDGF-BB), which is known to enhance hMSCs proliferation in human serum, was selected as a model growth factor, and biodegradable copolyester of PHBHHx was chosen to be the sustained release vehicle. PDGF-BB phospholipid complex encapsulated PHBHHx nanoparticles were fabricated, and their effect on hMSCs proliferation was investigated via assays of CCK-8 and live-dead staining to cells inoculated in 2D tissue culture plates and 3D collagen gel scaffolds, respectively. The resulting spherical PHBHHx nanoparticles were stable in terms of their mean particle size, polydispersity index and zeta potential before and after lyophilization. In vitro study revealed a sustained release of PDGF-BB with a low burst release. Furthermore, sustained released PDGF-BB was revealed to significantly promote hMSCs proliferation in both cell monolayer and cell seeded 3D collagen scaffolds inoculated in serum-free media. Therefore, the 3D collagen matrices with locally sustained release growth factor nanoparticles hold promise to be used for stem cell tissue engineering.
    Publisher
    Wiley Periodicals, Inc
    Journal
    Journal of Biomedical Materials Research Part A
    URI
    http://hdl.handle.net/2436/621531
    DOI
    10.1002/jbm.a.35149
    Additional Links
    https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.35149
    Type
    Journal article
    Language
    en
    ISSN
    15493296
    Sponsors
    HYANJI Scaffold Project (European Commission Framework 7 program: SP3 PEOPLE); contract grant number: PIRSESGA-2008-230791
    ae974a485f413a2113503eed53cd6c53
    10.1002/jbm.a.35149
    Scopus Count
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    Faculty of Science and Engineering

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