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dc.contributor.authorRajasekar, Adharsh
dc.contributor.authorSekar, Raju
dc.contributor.authorMedina-Roldán, Eduardo
dc.contributor.authorBridge, Jonathan
dc.contributor.authorMoy, Charles K S
dc.contributor.authorWilkinson, Stephen
dc.date.accessioned2018-06-19T14:10:42Z
dc.date.available2018-06-19T14:10:42Z
dc.date.issued2018-04-10
dc.identifier.citationRajasekar, A., Sekar R., Medina-Roldán E., Bridge, J., Moy, CKS., Wilkinson, S. (2018) 'Next-generation sequencing showing potential leachate influence on bacterial communities around a landfill in China', Canadian Journal of Microbiology, 64 (8) pp. 537-549. doi: 10.1139/cjm-2017-0543
dc.identifier.issn0008-4166
dc.identifier.pmid29633622
dc.identifier.doi10.1139/cjm-2017-0543
dc.identifier.urihttp://hdl.handle.net/2436/621342
dc.description.abstractThe impact of contaminated leachate on groundwater from landfills is well known, but the specific effects on bacterial consortia are less well-studied. Bacterial communities in a landfill and an urban site located in Suzhou, China, were studied using Illumina high-throughput sequencing. A total of 153 944 good-quality reads were produced and sequences assigned to 6388 operational taxonomic units. Bacterial consortia consisted of up to 16 phyla, including Proteobacteria (31.9%-94.9% at landfill, 25.1%-43.3% at urban sites), Actinobacteria (0%-28.7% at landfill, 9.9%-34.3% at urban sites), Bacteroidetes (1.4%-25.6% at landfill, 5.6%-7.8% at urban sites), Chloroflexi (0.4%-26.5% at urban sites only), and unclassified bacteria. Pseudomonas was the dominant (67%-93%) genus in landfill leachate. Arsenic concentrations in landfill raw leachate (RL) (1.11 × 103 μg/L) and fresh leachate (FL2) (1.78 × 103 μg/L) and mercury concentrations in RL (10.9 μg/L) and FL2 (7.37 μg/L) exceeded Chinese State Environmental Protection Administration standards for leachate in landfills. The Shannon diversity index and Chao1 richness estimate showed RL and FL2 lacked richness and diversity when compared with other samples. This is consistent with stresses imposed by elevated arsenic and mercury and has implications for ecological site remediation by bioremediation or natural attenuation.
dc.formatapplication/PDF
dc.language.isoen
dc.publisherCanadian Science Publishing
dc.relation.urlhttp://www.nrcresearchpress.com/doi/10.1139/cjm-2017-0543#.WykN_DTRVhE
dc.subjectlandfill
dc.subjectleachate
dc.subjectbacterial diversity
dc.subjectpseudomonas
dc.subjectarsenic
dc.titleNext-generation sequencing showing potential leachate influence on bacterial communities around a landfill in China
dc.typeJournal article
dc.identifier.journalCanadian Journal of Microbiology
dc.date.accepted2018-03-26
rioxxterms.funderinternal
rioxxterms.identifier.projectUOW19062018SW
rioxxterms.versionAM
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
rioxxterms.licenseref.startdate2018-04-10
dc.source.volume64
dc.source.issue8
dc.source.beginpage537
dc.source.endpage549
refterms.dateFCD2018-10-19T09:26:31Z
refterms.versionFCDAM
refterms.dateFOA2018-10-18T09:18:08Z
html.description.abstractThe impact of contaminated leachate on groundwater from landfills is well known, but the specific effects on bacterial consortia are less well-studied. Bacterial communities in a landfill and an urban site located in Suzhou, China, were studied using Illumina high-throughput sequencing. A total of 153 944 good-quality reads were produced and sequences assigned to 6388 operational taxonomic units. Bacterial consortia consisted of up to 16 phyla, including Proteobacteria (31.9%-94.9% at landfill, 25.1%-43.3% at urban sites), Actinobacteria (0%-28.7% at landfill, 9.9%-34.3% at urban sites), Bacteroidetes (1.4%-25.6% at landfill, 5.6%-7.8% at urban sites), Chloroflexi (0.4%-26.5% at urban sites only), and unclassified bacteria. Pseudomonas was the dominant (67%-93%) genus in landfill leachate. Arsenic concentrations in landfill raw leachate (RL) (1.11 × 103 μg/L) and fresh leachate (FL2) (1.78 × 103 μg/L) and mercury concentrations in RL (10.9 μg/L) and FL2 (7.37 μg/L) exceeded Chinese State Environmental Protection Administration standards for leachate in landfills. The Shannon diversity index and Chao1 richness estimate showed RL and FL2 lacked richness and diversity when compared with other samples. This is consistent with stresses imposed by elevated arsenic and mercury and has implications for ecological site remediation by bioremediation or natural attenuation.


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