Gene Loss and Lineage-Specific Restriction-Modification Systems Associated with Niche Differentiation in the Campylobacter jejuni Sequence Type 403 Clonal Complex
Authors
Morley, LauraMcNally, Alan
Paszkiewicz, Konrad
Corander, Jukka
Méric, Guillaume
Sheppard, Samuel K.
Blom, Jochen
Manning, Georgina
Issue Date
2015-05-05
Metadata
Show full item recordAbstract
ABSTRACT Campylobacter jejuni is a highly diverse species of bacteria commonly associated with infectious intestinal disease of humans and zoonotic carriage in poultry, cattle, pigs, and other animals. The species contains a large number of distinct clonal complexes that vary from host generalist lineages commonly found in poultry, livestock, and human disease cases to host-adapted specialized lineages primarily associated with livestock or poultry. Here, we present novel data on the ST403 clonal complex of C. jejuni , a lineage that has not been reported in avian hosts. Our data show that the lineage exhibits a distinctive pattern of intralineage recombination that is accompanied by the presence of lineage-specific restriction-modification systems. Furthermore, we show that the ST403 complex has undergone gene decay at a number of loci. Our data provide a putative link between the lack of association with avian hosts of C. jejuni ST403 and both gene gain and gene loss through nonsense mutations in coding sequences of genes, resulting in pseudogene formation.Citation
Morley, L., McNally, A., Paszkiewicz, K., Corander, J. et al (2015) Gene Loss and Lineage-Specific Restriction-Modification Systems Associated with Niche Differentiation in the Campylobacter jejuni Sequence Type 403 Clonal Complex, Applied and Environmental Microbiology, 81 (11), pp. 3641-3647Publisher
American Society of MicrobiologyJournal
Applied and Environmental MicrobiologyAdditional Links
http://aem.asm.org/lookup/doi/10.1128/AEM.00546-15Type
Journal articleLanguage
enDescription
This is an accepted manuscript of an article published by American Society for Microbiology in Applied and Environmental Microbiology on 05/05/2015, available online: https://doi.org/10.1128/AEM.00546-15 The accepted version of the publication may differ from the final published version.ISSN
0099-22401098-5336
Sponsors
L.M. was funded by a Nottingham Trent University Ph.D. studentship. A.M. is supported by the Royal Society (IE121459). S.K.S. is supported by a Wellcome Trust Career Development Fellowship, with additional funding from the BBSRC (BB/I02464X/1) and MRC-CLIMB (MR/L015080/1). J.C. is supported by ERC grant 239784 and AoF grant 251170. DNA sequencing was carried out at the Exeter Sequencing Service, which is supported by the following grants: Wellcome Trust Institutional Strategic Support Fund WT097835MF, Wellcome Trust Multi User Equipment Award WT101650MA, and BBSRC LOLA award BB/K003240/1.ae974a485f413a2113503eed53cd6c53
10.1128/AEM.00546-15