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    Electrospun Fibres of Polyhydroxybutyrate Synthesized by Ralstonia eutropha from Different Carbon Sources

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    Authors
    Irorere, Victor U.
    Bagheriasl, Soroosh
    Blevins, Mark
    Kwiecień, Iwona
    Stamboulis, Artemis
    Radecka, Iza
    Issue Date
    2014
    
    Metadata
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    Abstract
    The properties of PHB may be affected by the carbon source used in its production and this may affect nanofibres made from this polymer by electrospinning. In this study, P(3-HB) was produced from glucose, rapeseed oil, and olive oil by Ralstonia eutropha H16. Cell growth and polymer production were higher in olive or rapeseed oil supplemented media compared to glucose supplemented media. FT-IR, 1 H-, 13 C-NMR, and ESI/MS n confirmed that the synthesized polymers were P(3-HB). SEM micrograph showed the formation of nanofibres from P(3-HB) samples with the fibre diameters dependent on the source of the carbon used in polymer synthesis and the concentration of the polymer in the electrospinning solution. GPC showed that P(3-HB) from glucose (G-PHB) had a higher molecular weight ( 7.35 × 10 5  gmol −1 ) compared to P(3-HB) from rapeseed (R-PHB) and olive (O-PHB) oil. Differential scanning calorimetry (DSC) showed that the crystallinity of the electrospun polymers reduces with decreasing polymer concentration with R-PHB having lower crystallinity at all concentrations used. These observation shows that more PHB yield can be obtained using either rapeseed or olive oil compared to glucose with glucose producing polymers of higher molecular weight. It also show that electrospinning could be used to reduce the crystallinity of PHB fibres.
    Citation
    Electrospun Fibres of Polyhydroxybutyrate Synthesized by Ralstonia eutropha from Different Carbon Sources 2014, 2014:1 International Journal of Polymer Science
    Journal
    International Journal of Polymer Science
    URI
    http://hdl.handle.net/2436/620674
    DOI
    10.1155/2014/705359
    Additional Links
    http://www.hindawi.com/journals/ijps/2014/705359/
    Type
    Journal article
    Language
    en
    ISSN
    1687-9422
    1687-9430
    ae974a485f413a2113503eed53cd6c53
    10.1155/2014/705359
    Scopus Count
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    Faculty of Science and Engineering

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