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dc.contributor.authorPereira, Mayara P.
dc.contributor.authorFerreira, Laís A.A.
dc.contributor.authorda Silva, Flávia H.S.
dc.contributor.authorChristoffolete, Marcelo A.
dc.contributor.authorMetsios, George S.
dc.contributor.authorChaves, Valéria E.
dc.contributor.authorde França, Suélem A.
dc.contributor.authorDamazo, Amílcar S.
dc.contributor.authorFlouris, Andreas D.
dc.contributor.authorKawashita, Nair H.
dc.date.accessioned2017-09-19T14:44:51Z
dc.date.available2017-09-19T14:44:51Z
dc.date.issued2017-05-31
dc.identifier.citationPereira MP., Ferreira LAA., da Silva FHS., Christoffolete MA., Metsios GS., Chaves VE., de França SA., Damazo AS., Flouris AD., Kawashita NH. (2017) 'A low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue', Nutrition, 42 (October 2017), pp. 37-45
dc.identifier.issn0899-9007
dc.identifier.doi10.1016/j.nut.2017.05.007
dc.identifier.urihttp://hdl.handle.net/2436/620669
dc.description.abstractObjective The aim of this study was to evaluate the browning and origin of fatty acids (FAs) in the maintenance of triacylglycerol (TG) storage and/or as fuel for thermogenesis in perirenal adipose tissue (periWAT) and inguinal adipose tissue (ingWAT) of rats fed a low-protein, high-carbohydrate (LPHC) diet. Methods LPHC (6% protein, 74% carbohydrate) or control (C; 17% protein, 63% carbohydrate) diets were administered to rats for 15 d. The tissues were stained with hematoxylin and eosin for histologic analysis. The content of uncoupling protein 1 (UCP1) was determined by immunofluorescence. Levels of T-box transcription factor (TBX1), PR domain containing 16 (PRDM16), adipose triacylglycerol lipase (ATGL), hormone-sensitive lipase, lipoprotein lipase (LPL), glycerokinase, phosphoenolpyruvate carboxykinase (PEPCK), glucose transporter 4, β3-adrenergic receptor (AR), β1-AR, protein kinase A (PKA), adenosine-monophosphate-activated protein kinase (AMPK), and phospho-AMPK were determined by immunoblotting. Serum fibroblast growth factor 21 (FGF21) was measured using a commercial kit (Student's t tests, P < 0.05). Results The LPHC diet increased FGF21 levels by 150-fold. The presence of multilocular adipocytes, combined with the increased contents of UCP1, TBX1, and PRDM16 in periWAT of LPHC-fed rats, suggested the occurrence of browning. The contents of β1-AR and LPL were increased in the periWAT. The ingWAT showed higher ATGL and PEPCK levels, phospho-AMPK/AMPK ratio, and reduced β3-AR and PKA levels. Conclusion These findings suggest that browning occurred only in the periWAT and that higher utilization of FAs from blood lipoproteins acted as fuel for thermogenesis. Increased glycerol 3-phosphate generation by glyceroneogenesis increased FAs reesterification from lipolysis, explaining the increased TG storage in the ingWAT.
dc.description.sponsorshipIRSES FP7
dc.formatapplication/pdf
dc.language.isoen
dc.publisherElsevier
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S089990071730103X
dc.subjectBrite
dc.subjectEnergy expenditure
dc.subjectHigh-carbohydrate diet
dc.subjectLow-protein
dc.subjectProtein restriction
dc.subjectWhite adipose tissue
dc.titleA low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue
dc.typeJournal article
dc.identifier.journalNutrition
dc.date.accepted2017-05-17
rioxxterms.funderEuropean Social Fund and Greek national funds
rioxxterms.identifier.projectUoW190917GM
dc.source.volume42
dc.source.issueOctober 2017
dc.source.beginpage37
dc.source.endpage45
refterms.dateFCD2018-10-19T09:12:35Z
refterms.dateFOA2018-05-31T00:00:00Z


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