• Admin Login
    View Item 
    •   Home
    • Faculty of Education, Health and Wellbeing
    • Faculty of Education, Health and Wellbeing
    • View Item
    •   Home
    • Faculty of Education, Health and Wellbeing
    • Faculty of Education, Health and Wellbeing
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WIRECommunitiesTitleAuthorsIssue DateSubmit DateSubjectsTypesJournalDepartmentPublisherThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsTypesJournalDepartmentPublisher

    Administrators

    Admin Login

    Local Links

    AboutThe University LibraryOpen Access Publications PolicyDeposit LicenceCOREWIRE Copyright and Reuse Information

    Statistics

    Display statistics

    A low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Thumbnail
    Name:
    Pereira_M_P_A_low-protein_2017.pdf
    Size:
    1.838Mb
    Format:
    PDF
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Pereira, Mayara P.
    Ferreira, Laís A.A.
    da Silva, Flávia H.S.
    Christoffolete, Marcelo A.
    Metsios, George S.
    Chaves, Valéria E.
    de França, Suélem A.
    Damazo, Amílcar S.
    Flouris, Andreas D.
    Kawashita, Nair H.
    Issue Date
    2017-05-31
    
    Metadata
    Show full item record
    Abstract
    Objective The aim of this study was to evaluate the browning and origin of fatty acids (FAs) in the maintenance of triacylglycerol (TG) storage and/or as fuel for thermogenesis in perirenal adipose tissue (periWAT) and inguinal adipose tissue (ingWAT) of rats fed a low-protein, high-carbohydrate (LPHC) diet. Methods LPHC (6% protein, 74% carbohydrate) or control (C; 17% protein, 63% carbohydrate) diets were administered to rats for 15 d. The tissues were stained with hematoxylin and eosin for histologic analysis. The content of uncoupling protein 1 (UCP1) was determined by immunofluorescence. Levels of T-box transcription factor (TBX1), PR domain containing 16 (PRDM16), adipose triacylglycerol lipase (ATGL), hormone-sensitive lipase, lipoprotein lipase (LPL), glycerokinase, phosphoenolpyruvate carboxykinase (PEPCK), glucose transporter 4, β3-adrenergic receptor (AR), β1-AR, protein kinase A (PKA), adenosine-monophosphate-activated protein kinase (AMPK), and phospho-AMPK were determined by immunoblotting. Serum fibroblast growth factor 21 (FGF21) was measured using a commercial kit (Student's t tests, P < 0.05). Results The LPHC diet increased FGF21 levels by 150-fold. The presence of multilocular adipocytes, combined with the increased contents of UCP1, TBX1, and PRDM16 in periWAT of LPHC-fed rats, suggested the occurrence of browning. The contents of β1-AR and LPL were increased in the periWAT. The ingWAT showed higher ATGL and PEPCK levels, phospho-AMPK/AMPK ratio, and reduced β3-AR and PKA levels. Conclusion These findings suggest that browning occurred only in the periWAT and that higher utilization of FAs from blood lipoproteins acted as fuel for thermogenesis. Increased glycerol 3-phosphate generation by glyceroneogenesis increased FAs reesterification from lipolysis, explaining the increased TG storage in the ingWAT.
    Citation
    Pereira MP., Ferreira LAA., da Silva FHS., Christoffolete MA., Metsios GS., Chaves VE., de França SA., Damazo AS., Flouris AD., Kawashita NH. (2017) 'A low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue', Nutrition, 42 (October 2017), pp. 37-45
    Publisher
    Elsevier
    Journal
    Nutrition
    URI
    http://hdl.handle.net/2436/620669
    DOI
    10.1016/j.nut.2017.05.007
    Additional Links
    http://linkinghub.elsevier.com/retrieve/pii/S089990071730103X
    Type
    Journal article
    Language
    en
    ISSN
    0899-9007
    Sponsors
    IRSES FP7
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.nut.2017.05.007
    Scopus Count
    Collections
    Faculty of Education, Health and Wellbeing

    entitlement

     

    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.