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dc.contributor.authorRadecka, Iza
dc.contributor.authorIrorere, Victor
dc.contributor.authorJiang, Guozhan
dc.contributor.authorHill, David
dc.contributor.authorWilliams, Craig
dc.contributor.authorAdamus, Grazyna
dc.contributor.authorKwiecień, Michal
dc.contributor.authorMarek, Adam
dc.contributor.authorZawadiak, Jan
dc.contributor.authorJohnston, Brian
dc.contributor.authorKowalczuk , Marek
dc.date.accessioned2016-07-21T08:36:48Z
dc.date.available2016-07-21T08:36:48Z
dc.date.issued2016-05-13
dc.identifier.citationRadecka I., Irorere V., Jiang G., Hill D., Williams C., Adamus G., Kwiecień M., Marek AA., Zawadiak J., Johnston B., Kowalczuk M. (2016) 'Oxidized polyethylene wax as a potential carbon source for PHA production', Materials, 9 (5): 367
dc.identifier.issn1996-1944
dc.identifier.doi10.3390/ma9050367
dc.identifier.urihttp://hdl.handle.net/2436/617298
dc.description© 2016 The Authors. Published by MDPI. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3390/ma9050367
dc.description.abstractWe report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.
dc.language.isoen
dc.publisherMDPI - Open Access Publishing
dc.relation.urlhttp://www.mdpi.com/1996-1944/9/5/367
dc.subjectpolyhdyroxyalkanoates
dc.subjectoxidized PE wax
dc.subjectmedium chain length PHA (mcl-PHA)
dc.subjectRalstonia eutropha H16
dc.subjectmass spectrometry
dc.titleOxidized polyethylene wax as a potential carbon source for PHA production
dc.typeJournal article
dc.identifier.journalMaterials
dc.identifier.articlenumber367
dc.date.accepted2016-05-09
rioxxterms.funderJisc
rioxxterms.identifier.projectBIOCLEAN Project, Contract No. 312100
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/CC BY-NC-ND 4.0
rioxxterms.licenseref.startdate2016-07-21
dc.source.volume9
dc.source.issue5
refterms.dateFCD2018-10-19T09:26:31Z
refterms.versionFCDVoR
refterms.dateFOA2016-07-21T00:00:00Z
html.description.abstractWe report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.


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