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    Inactivation of acanthamoeba spp. and other ocular pathogens by application of cold atmospheric gas plasma

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    Authors
    Heaselgrave, Wayne
    Shama, Gilbert
    Andrew, Peter W
    Kong, Michael G
    Issue Date
    2016-05-02
    
    Metadata
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    Abstract
    Currently there are estimated to be approximately 3.7 million contact lens wearers in the United Kingdom and 39.2 million in North America. Contact lens wear is a major risk factor for developing an infection of the cornea known as keratitis due to poor lens hygiene practices. While there is an international standard for testing disinfection methods against bacteria and fungi (ISO 14729), no such guidelines exist for the protozoan Acanthamoeba, which causes a potentially blinding keratitis most commonly seen in contact lens wearers, and as a result, many commercially available disinfecting solutions show incomplete disinfection after 6 and 24 h of exposure. Challenge test assays based on international standard ISO 14729 were used to determine the antimicrobial activity of cold atmospheric gas plasma (CAP) against Pseudomonas aeruginosa, Candida albicans, and trophozoites and cysts of Acanthamoeba polyphaga and Acanthamoeba castellanii P. aeruginosa and C. albicans were completely inactivated in 0.5 min and 2 min, respectively, and trophozoites of A. polyphaga and A. castellanii were completely inactivated in 1 min and 2 min, respectively. Furthermore, for the highly resistant cyst stage of both species, complete inactivation was achieved after 4 min of exposure to CAP. This study demonstrates that the CAP technology is highly effective against bacterial, fungal, and protozoan pathogens. The further development of this technology has enormous potential, as this approach is able to deliver the complete inactivation of ocular pathogens in minutes, in contrast to commercial multipurpose disinfecting solutions that require a minimum of 6 h.
    Citation
    Heaselgrave, W., Shama, G., Andrew, PW., Kong, MG. 'Inactivation of Acanthamoeba spp. and Other Ocular Pathogens by Application of Cold Atmospheric Gas Plasma', Applied Environmental Microbiology, 82 (10) pp. 3143-8
    Publisher
    American Society for Microbiology
    Journal
    Applied and Environmental Microbiology
    URI
    http://hdl.handle.net/2436/611712
    DOI
    10.1128/AEM.03863-15
    PubMed ID
    26994079
    Additional Links
    http://aem.asm.org/content/82/10.toc
    Type
    Journal article
    Language
    en
    ISSN
    1098-5336
    ae974a485f413a2113503eed53cd6c53
    10.1128/AEM.03863-15
    Scopus Count
    Collections
    Faculty of Science and Engineering

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