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Validation of the Polar RS800CX for assessing heart rate variability during rest, moderate cycling and post-exercise recoveryBackground: Heart rate variability (HRV) is an autonomic nervous system marker that provides reliable information for both disease prevention and diagnosis; it is also used in sport settings. We examined the validity of the Polar RS800CX heart rate monitor during rest, moderate cycling, and recovery in considering the total of 24 HRV indices. Method: A total of 32 healthy males (age=24.78±6.87 years, body mass index=24.48±3.13 kg/m 2 ) completed a session comprised by three 20-minute time periods of resting, cycling at 60% of maximal heart rate, and recovery using a Polar RS800CX and an electrocardiogram (ECG) monitors. The HRV indices included time-domain, frequency-domain, Poincaré plot and recurrence plot. Bland–Altman plot analysis was used to estimate agreement between Polar RS800CX and ECG. Results: We detected significant associations (r>0.75, p<0.05) in all HRV indices, while five out of 24 HRV indices displayed significant mean differences (p<0.05) between Polar RS800CX and ECG during the resting period. However, for the exercise and recovery periods, we found significant mean differences (p<0.05) in 16/24 and 22/24 HRV indices between the two monitors, respectively. Conclusion: It is concluded that Polar RS800CX is a valid tool for monitoring HRV in individuals at resting conditions, but it displays inconsistency when used during exercise at 60% of maximal heart rate and recovery periods.