• Relationship between Type 2 Diabetes and Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency and Their Effect on Oxidative Stress

      Engwa, Godwill Azeh; Nwalo, Friday Nweke; Chibuzor, Gregory Eze; Ejiagha, Endaline Chiamaka; Abonyi, Micheal Chinweuba; Ugwu, Theophilus Ejiofor; Ikechukwu Obiudu, Kenneth; Agbafor, Kingsley Nwonu; Oyejide Ojo, Opeolu; Ewa Ubi, Benjamin (OMICS Publishing Group, 2018-08-20)
      Objective: Though the relationship between glucose-6-phosphate dehydrogenase (G6PD) deficiency and type 2 diabetes (T2D) has been reported, their interaction to influence oxidative stress is not fully understood. This study was conducted to investigate the relationship between T2D and G6PD deficiency and assessed their effect on oxidative stress. Methodology: A total of 73 T2D and 75 non-diabetic (ND) out-patients at Enugu State University Teaching Hospital (ESUTH) in Enugu, Nigeria were recruited. They were screened for G6PD deficiency, oxidative stress markers; lipid peroxidation, protein peroxidation and glycated haemoglobin (HbA1c) and antioxidant enzymes; superoxide dismutase (SOD) and catalase activities were assayed. Results: Oxidative stress was influenced by T2D as malondialdehyde (MDA), protein carbonyl and HbA1c levels were significantly higher (p<0.05) in T2D patients compared to ND patients. Significantly (p<0.05) decreased SOD and catalase activity were also observed in T2D patients. G6PD deficiency and T2D showed a significant relationship (p<0.05) in male patients (χ 2=5.070; p=0.024). Moreover, there was no significant (p>0.05) interaction between G6PD deficiency and T2D to influence oxidative stress in patients. Conclusion: The interaction between G6PD deficiency and T2D did not influence oxidative stress though there was a possible relationship between G6PD deficiency and T2D in male patients.
    • Visualisation of latent fingerprint on wild bird eggshells by alternate light sources following superglue fuming

      Darby, Alison; Rogers, Christopher; Greene, Benjamin; Parry, Eleri; Wray, Elisabeth M.; Yang, Jixin (OMICS Publishing Group, 2015-05-20)
      The theft of the eggs of endangered or protected species of bird, and subsequent reduction in wildlife population, is a significant problem worldwide. Detection rates are comparatively low towards this type of crime and fingerprinting of egg shells is infrequently utilised due to the technical barrier. This paper explores a novel technique using cyanoacrylate (superglue) fuming in conjunction with fluorescent dye to visualise latent fingerprints upon avian eggshells assisted with alternate light sources. A systematic investigation of experimental parameters has also been carried out to optimise the condition for the fingerprint visualisation. This research project has successfully developed latent fingerprints upon smooth wild bird eggshells but was less successful on developing prints on more textured, porous eggshells.