• Admin Login
    View Item 
    •   Home
    • Research Institute in Healthcare Science
    • Research Institute in Healthcare Science
    • View Item
    •   Home
    • Research Institute in Healthcare Science
    • Research Institute in Healthcare Science
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WIRECommunitiesTitleAuthorsIssue DateSubmit DateSubjectsTypesJournalDepartmentPublisherThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsTypesJournalDepartmentPublisher

    Administrators

    Admin Login

    Local Links

    AboutThe University LibraryOpen Access Publications PolicyDeposit LicenceCOREWIRE Copyright and Reuse Information

    Statistics

    Display statistics

    Mitoparan and target-selective chimeric analogues: membrane translocation and intracellular redistribution induces mitochondrial apoptosis.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Authors
    Jones, Sarah
    Martel, Cecile
    Belzacq-Casagrande, Anne-Sophie
    Brenner, Catherine
    Howl, John D.
    Issue Date
    2008
    
    Metadata
    Show full item record
    Abstract
    Mastoparan, and structurally-related amphipathic peptides, may induce cell death by augmentation of necrotic and/or apoptotic pathways. To more precisely delineate cytotoxic mechanisms, we determined that [Lys(5,8)Aib(10)]mastoparan (mitoparan) specifically induces apoptosis of U373MG and ECV304 cells, as demonstrated by endonuclease and caspase-3 activation and phosphatidylserine translocation. Live cell imaging confirmed that, following translocation of the plasma membrane, mitoparan specifically co-localizes with mitochondria. Complementary studies indicated that mitoparan induces swelling and permeabilization of isolated mitochondria, through cooperation with a protein of the permeability transition pore complex VDAC, leading to the release of the apoptogenic factor, cytochrome c. N-terminal acylation of mitoparan facilitated the synthesis of chimeric peptides that incorporated target-specific address motifs including an integrin-specific RGD sequence and a Fas ligand mimetic. Significantly, these sychnologically-organised peptides demonstrated further enhanced cytotoxic potencies. We conclude that the cell penetrant, mitochondriotoxic and apoptogenic properties of mitoparan, and its chimeric analogues, offer new insights to the study and therapeutic induction of apoptosis.
    Citation
    Biochimica et Biophysica Acta - Molecular Cell Research, 1783(5): 849-863
    Publisher
    Amsterdam: Elsevier
    Journal
    Biochimica et Biophysica Acta - Molecular Cell Research
    URI
    http://hdl.handle.net/2436/30272
    DOI
    10.1016/j.bbamcr.2008.01.009
    PubMed ID
    18267123
    Additional Links
    http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T20-4RP0MF4-1&_user=10&_coverDate=05%2F31%2F2008&_rdoc=17&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234904%232008%23982169994%23686190%23FLA%23display%23Volume)&_cdi=4904&_sort=d&_docanchor=&_ct=26&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=78ecb320a945265feb7eb85cf01981e6
    Type
    Journal article
    Language
    en
    ISSN
    0006-3002
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bbamcr.2008.01.009
    Scopus Count
    Collections
    Research Institute in Healthcare Science

    entitlement

    Related articles

    • Enantiomer-specific bioactivities of peptidomimetic analogues of mastoparan and mitoparan: characterization of inverso mastoparan as a highly efficient cell penetrating peptide.
    • Authors: Jones S, Howl J
    • Issue date: 2012 Jan 18
    • The cationic tetradecapeptide mastoparan as a privileged structure for drug discovery: Enhanced antimicrobial properties of mitoparan analogues modified at position-14.
    • Authors: Howl J, Howl L, Jones S
    • Issue date: 2018 Mar
    • Mastoparan peptide causes mitochondrial permeability transition not by interacting with specific membrane proteins but by interacting with the phospholipid phase.
    • Authors: Yamamoto T, Ito M, Kageyama K, Kuwahara K, Yamashita K, Takiguchi Y, Kitamura S, Terada H, Shinohara Y
    • Issue date: 2014 Sep
    • Modulation of mitochondrial activity in HaCaT keratinocytes by the cell penetrating peptide Z-Gly-RGD(DPhe)-mitoparan.
    • Authors: Richardson A, Muir L, Mousdell S, Sexton D, Jones S, Howl J, Ross K
    • Issue date: 2018 Jan 30
    • Inflammation and apoptosis induced by mastoparan Polybia-MPII on skeletal muscle.
    • Authors: Rocha T, de Barros LL, Fontana K, de Souza BM, Palma MS, da Cruz-Höfling MA
    • Issue date: 2010 Jun 15
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.