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dc.contributor.authorSmith, K.A.
dc.contributor.authorNelson, Paul N.
dc.contributor.authorWarren, Phil
dc.contributor.authorAstley, S.J.
dc.contributor.authorMurray, Paul G.
dc.contributor.authorGreenman, J.
dc.date.accessioned2008-06-19T15:21:00Z
dc.date.available2008-06-19T15:21:00Z
dc.date.issued2004
dc.identifier.citationJournal of Clinical Pathology, 57(9): 912-917
dc.identifier.issn0021-9746
dc.identifier.pmid15333649
dc.identifier.doi10.1136/jcp.2003.014407
dc.identifier.urihttp://hdl.handle.net/2436/30203
dc.description.abstractRecombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanized antibodies is given, together with details of the generation of antibody fragments--for example, single chain Fv fragments. Phage antibody fragments are fast becoming popular and can be generated by simple established methods of affinity enrichment from libraries derived from immune cells. Phage display methodology can also be used for the affinity enrichment of existing antibody fragments to provide a reagent with a higher affinity. Here, phage antibodies are demystified to provide a greater understanding of the potential of these reagents and to engage clinicians and biomedical scientists alike to think about potential applications in pathology and clinical settings.
dc.language.isoen
dc.publisherBMJ Publishing Group Ltd.
dc.relation.urlhttp://jcp.bmj.com/cgi/content/abstract/57/9/912
dc.subjectPhage display
dc.subjectMonoclonal antibodies
dc.subjectMolecular Biology
dc.subject.meshAnimals
dc.subject.meshAntibodies, Monoclonal
dc.subject.meshFlow Cytometry
dc.subject.meshHumans
dc.subject.meshImmunoglobulin Fragments
dc.subject.meshImmunohistochemistry
dc.subject.meshPeptide Library
dc.subject.meshRecombinant Proteins
dc.titleDemystified...recombinant antibodies.
dc.typeJournal article
dc.identifier.journalJournal of Clinical Pathology
html.description.abstractRecombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanized antibodies is given, together with details of the generation of antibody fragments--for example, single chain Fv fragments. Phage antibody fragments are fast becoming popular and can be generated by simple established methods of affinity enrichment from libraries derived from immune cells. Phage display methodology can also be used for the affinity enrichment of existing antibody fragments to provide a reagent with a higher affinity. Here, phage antibodies are demystified to provide a greater understanding of the potential of these reagents and to engage clinicians and biomedical scientists alike to think about potential applications in pathology and clinical settings.


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