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dc.contributor.authorHowl, John D.
dc.date.accessioned2008-06-12T08:31:37Z
dc.date.available2008-06-12T08:31:37Z
dc.date.issued2005
dc.identifier.citationMethods in Molecular Biology, 298
dc.identifier.isbn978-1-58829-317-6
dc.identifier.isbn978-1-59259-877-9
dc.identifier.issn1064-3745
dc.identifier.doi10.1385/1592598773
dc.identifier.urihttp://hdl.handle.net/2436/29934
dc.descriptionThis title is published as a book but is also in a journal series
dc.description.abstractHands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. (Humana Press)
dc.language.isoen
dc.publisherClifton, N.J.: Humana Press
dc.relation.urlhttps://www.springer.com/gp/book/9781588293176
dc.subjectSynthesis
dc.subjectLigands
dc.subjectMolecular Biology
dc.subjectMolecular Sequence Data
dc.subjectPeptides
dc.subjectGenomics
dc.titlePeptide Synthesis and Applications
dc.typeAuthored book
dc.identifier.journalMethods in Molecular Biology
html.description.abstractHands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. (Humana Press)


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