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    Simultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum Chuanxiong using HPLC-DAD-MS.

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    Chemical & Pharmaceutical Bulletin, ...
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    Authors
    Yi, Tao
    Leung, Kelvin Sze-Yin
    Lu, Guang-Hua
    Chan, Kelvin C.
    Zhang, Hao
    Issue Date
    2006
    
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    Abstract
    An HPLC-DAD-MS method was developed for the qualitative and quantitative analysis of the major constituents in Chuanxiong (the dried rhizome of Ligusticum chuanxiong Hort). Twenty compounds including phenolic constituents, alkylphthalides and phthalide dimers were identified using online ESI-MS and comparisons with literature data and standard compounds, and six of them were quantified by HPLC-DAD simultaneously. A comprehensive validation of the method including sensitivity, linearity, repeatability and recovery was conducted. The linear regressions were acquired with R(2) > 0.99 and limit of detection (LOD, S/N = 3) values were between 1.5 and 2.5 ng. The repeatability was evaluated by intra- and inter-day assays, and relative standard deviation (RSD) values were reported within 1.87%. The recovery studies for the quantified compounds were observed in the range of 96.36-102.37% with RSD values less than 2.63%. These phenolic constituents and alkylphthalides, the major constituents in Chuanxiong, are generally regarded as the index for the quality assessment of this herb. The overall procedure is accurate and reproducible, which is considered suitable for the qualitative and quantitative analyses of a large number of Chuanxiong samples.
    Citation
    Chemical & Pharmaceutical Bulletin, 54(2): 255-259
    Publisher
    Tokyo: The Pharmaceutical Society of Japan
    Journal
    Chemical & Pharmaceutical Bulletin
    URI
    http://hdl.handle.net/2436/29757
    PubMed ID
    16462078
    Additional Links
    http://www.jstage.jst.go.jp/article/cpb/54/2/54_255/_article
    Type
    Journal article
    Language
    en
    ISSN
    0009-2363
    1347-5223
    Collections
    Research Institute in Healthcare Science

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