Determination of oleanolic acid in Caulis clematidis armandii by HPLC

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Authors
Qing, Lin-Sen
Zhang, Hao
Lu, Guang-Hua
Chan, Kelvin C.
Leung, Kelvin Sze-Yin
Chen Chu, Sze-Yin
Issue Date
2006

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Abstract
OBJECTIVE: To develop an HPLC method for the content determination of oleanolic acid in Caulis clematidis armandii after hydrolysis. METHODS: In the system of HPLC, a Alltima C_(18)(250 mm×4.6 mm,5 μm) column was used with a mixture of methanol-water-acetic acid-triethylamine(89∶11∶0.04∶0.02) as the mobile phase.The measuring wavelength was set at 207 nm.The flow rate was 1.0 ml·min~(-1) and the column temperature maintained at 45 ℃.RESULTS The oleanolic acid peak in chromatograms was completely separated from ...
Citation
West China Journal of Pharmaceutical Sciences, 21(3): 273-274
Publisher
ILIB
Journal
West China Journal of Pharmaceutical Sciences
URI
http://hdl.handle.net/2436/29755
Additional Links
http://caod.oriprobe.com/articles/10605571/Determination_of_oleanolic_acid_in_Caulis_clematidis_armandii_by_HPLC.htm
Type
Journal article
Language
zh
ISSN
1006-0103
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