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dc.contributor.authorCui, Liang
dc.contributor.authorChan, Wan
dc.contributor.authorWu, Jian-Lin
dc.contributor.authorJiang, Zhi-Hong
dc.contributor.authorChan, Kelvin C.
dc.contributor.authorCai, Zongwei
dc.date.accessioned2008-06-10T09:31:43Z
dc.date.available2008-06-10T09:31:43Z
dc.date.issued2008
dc.identifier.citationTalanta, 75(4): 1002-1007
dc.identifier.issn00399140
dc.identifier.doi10.1016/j.talanta.2007.12.045
dc.identifier.urihttp://hdl.handle.net/2436/29754
dc.description.abstractMetabolism and pharmacokinetic studies on rat were conducted for lithospermic acid B, one of the components from Radix Salviae Miltiorrhizae (danshen) that shows many bioactivities. Liquid chromatography–electrospray ionization mass spectrometry method was applied for the determination of lithospermic acid B and its metabolites in samples from in vitro and in vivo metabolism studies. Rat plasma samples collected after intravenous administration were analyzed for obtaining pharmacokinetic data of lithospermic acid B. Four O-methylated metabolites, namely one monomethyl-, two dimethyl- and one trimethyl-lithospermic acid B, were detected when lithospermic acid B was incubated in rat hepatic cytosol. These four metabolites were also detected in rat bile, plasma and feces samples after intravenous administration of lithospermic acid B. The in vitro and in vivo results indicate that the methylation is the main metabolic pathway of lithospermic acid B. The danshen component and its methylated metabolites were excreted to rat bile and feces. (Elsevier)
dc.language.isoen
dc.publisherAmsterdam: Elsevier
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-4RKMHW4-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ae9ff64b23dc55ac9e68d5a60cb0bb1d
dc.subjectLithospermic acid B
dc.subjectDrug metabolism
dc.subjectPharmacokinetics
dc.subjectRats
dc.subjectDanshen
dc.subjectChromatography, High Performance Liquid
dc.subjectHaematology, animal
dc.subjectHPLC
dc.titleHigh Performance liquid chromatography-mass spectrometry analysis for rat metabolism and pharmacokinetic studies of lithospermic acid B from danshen
dc.typeJournal article
dc.identifier.journalTalanta
html.description.abstractMetabolism and pharmacokinetic studies on rat were conducted for lithospermic acid B, one of the components from Radix Salviae Miltiorrhizae (danshen) that shows many bioactivities. Liquid chromatography–electrospray ionization mass spectrometry method was applied for the determination of lithospermic acid B and its metabolites in samples from in vitro and in vivo metabolism studies. Rat plasma samples collected after intravenous administration were analyzed for obtaining pharmacokinetic data of lithospermic acid B. Four O-methylated metabolites, namely one monomethyl-, two dimethyl- and one trimethyl-lithospermic acid B, were detected when lithospermic acid B was incubated in rat hepatic cytosol. These four metabolites were also detected in rat bile, plasma and feces samples after intravenous administration of lithospermic acid B. The in vitro and in vivo results indicate that the methylation is the main metabolic pathway of lithospermic acid B. The danshen component and its methylated metabolites were excreted to rat bile and feces. (Elsevier)


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