The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.
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Authors
Ejtehadi, H. DavariFreimanis, Graham L.
Ali, H.A.
Bowman, S.J.
Alavi, A.
Axford, J.
Callaghan, R.
Nelson, Paul N.
Issue Date
2006
Metadata
Show full item recordAbstract
OBJECTIVE: To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease. DESIGN: A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis. METHODS: 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products. RESULTS: Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis (p = 0.01) or in the healthy controls (p = 0.02). CONCLUSION: There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.Citation
Annals of the Rheumatic Diseases, 65 (5): 612-616Publisher
BMJ PublishingJournal
Annals of the Rheumatic DiseasesPubMed ID
16192292Additional Links
http://ard.bmj.com/cgi/content/full/65/5/612Type
Journal articleLanguage
enISSN
0003-4967ae974a485f413a2113503eed53cd6c53
10.1136/ard.2004.031146
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