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    The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.

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    Authors
    Ejtehadi, H. Davari
    Freimanis, Graham L.
    Ali, H.A.
    Bowman, S.J.
    Alavi, A.
    Axford, J.
    Callaghan, R.
    Nelson, Paul N.
    Issue Date
    2006
    
    Metadata
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    Abstract
    OBJECTIVE: To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease. DESIGN: A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis. METHODS: 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products. RESULTS: Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis (p = 0.01) or in the healthy controls (p = 0.02). CONCLUSION: There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.
    Citation
    Annals of the Rheumatic Diseases, 65 (5): 612-616
    Publisher
    BMJ Publishing
    Journal
    Annals of the Rheumatic Diseases
    URI
    http://hdl.handle.net/2436/29461
    DOI
    10.1136/ard.2004.031146
    PubMed ID
    16192292
    Additional Links
    http://ard.bmj.com/cgi/content/full/65/5/612
    Type
    Journal article
    Language
    en
    ISSN
    0003-4967
    ae974a485f413a2113503eed53cd6c53
    10.1136/ard.2004.031146
    Scopus Count
    Collections
    Research Institute in Healthcare Science

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