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dc.contributor.authorBates, Ruth L.
dc.contributor.authorFrampton, Geoffrey
dc.contributor.authorRose, Marlene L.
dc.contributor.authorMurphy, John J.
dc.date.accessioned2008-06-04T09:30:42Z
dc.date.available2008-06-04T09:30:42Z
dc.date.issued2003
dc.identifier.citationTransplantation, 75(8): 1347-1350
dc.identifier.issn0041-1337
dc.identifier.pmid12717228
dc.identifier.doi10.1097/01.TP.0000061790.08550.EC
dc.identifier.urihttp://hdl.handle.net/2436/29437
dc.description.abstractBACKGROUND: Antibodies to endothelial derived non-human leukocyte antigens (HLA) have been associated with transplant (Tx)-associated coronary artery disease (CAD) after cardiac transplantation; however, few have been identified. The aim of this study was to screen a human coronary artery endothelial cell cDNA library with patient sera to establish the diversity and nature of the target antigens. METHODS: A human coronary artery endothelial cell cDNA library was screened with sera from seven long-term cardiac transplant patients with angiographically diagnosed TxCAD and sera from five healthy volunteers. RESULTS: Of the seven patients' sera, five showed reactivity, as did sera from two of the five normal subjects. Eighteen positive cDNA clones were isolated by TxCAD sera; DNA sequence analysis and DNA database searching identified all but one clone; 16 were nuclear or cytoplasmic proteins and 1 of them was the cell surface protein neuropilin 2. Five clones were targeted by normal sera. A different spectrum of reactive clones was identified by the sera of each patient where reactive clones were evident. CONCLUSIONS: A high diversity of non-HLA antigens, probably autoantigens, are involved in the pathogenesis of TxCAD.
dc.language.isoen
dc.publisherLippincott Williams & Wilkins
dc.relation.urlhttp://www.transplantjournal.com/pt/re/transplantation/abstract.00007890-200304270-00057.htm
dc.subject.meshAdult
dc.subject.meshAntigens
dc.subject.meshCoronary Disease
dc.subject.meshCytoplasm
dc.subject.meshGene Library
dc.subject.meshHeart Transplantation
dc.subject.meshHumans
dc.subject.meshMiddle Aged
dc.subject.meshNeuropilin-2
dc.subject.meshNuclear Proteins
dc.subject.meshProteins
dc.subject.meshReference Values
dc.titleHigh diversity of non-human leukocyte antigens in transplant-associated coronary artery disease.
dc.typeArticle
dc.identifier.journalTransplantation
html.description.abstractBACKGROUND: Antibodies to endothelial derived non-human leukocyte antigens (HLA) have been associated with transplant (Tx)-associated coronary artery disease (CAD) after cardiac transplantation; however, few have been identified. The aim of this study was to screen a human coronary artery endothelial cell cDNA library with patient sera to establish the diversity and nature of the target antigens. METHODS: A human coronary artery endothelial cell cDNA library was screened with sera from seven long-term cardiac transplant patients with angiographically diagnosed TxCAD and sera from five healthy volunteers. RESULTS: Of the seven patients' sera, five showed reactivity, as did sera from two of the five normal subjects. Eighteen positive cDNA clones were isolated by TxCAD sera; DNA sequence analysis and DNA database searching identified all but one clone; 16 were nuclear or cytoplasmic proteins and 1 of them was the cell surface protein neuropilin 2. Five clones were targeted by normal sera. A different spectrum of reactive clones was identified by the sera of each patient where reactive clones were evident. CONCLUSIONS: A high diversity of non-HLA antigens, probably autoantigens, are involved in the pathogenesis of TxCAD.


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