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Variation between two near isogenic barley (Hordeum vulgare) cultivars in expression of the B subunit of the vacuolar ATPase in response to salinity.

dc.contributor.authorWei, Wenxue
dc.contributor.authorBilsborrow, Paul E.
dc.contributor.authorHooley, Paul
dc.contributor.authorFincham, Daron A.
dc.contributor.authorForster, Brian P.
dc.date.accessioned2008-04-03T09:12:33Z
dc.date.available2008-04-03T09:12:33Z
dc.date.issued2001
dc.identifier.citationHereditas, 135 (2-3): 227-231
dc.identifier.issn0018-0661
dc.identifier.pmid12152339
dc.identifier.urihttp://hdl.handle.net/2436/22193
dc.description.abstractA gene encoding the barley vacuolar ATPase subunit B (BSVAP) was differentially expressed between two near isogenic barley cultivars, Golden Promise and Maythorpe. This gene (BSVAP) was isolated by the mRNA differential display technique (DDRT-PCR). BSVAP was salt inducible under long-term salinity stress in the salt sensitive cultivar Maythorpe but less so in the relatively salt tolerant Golden Promise and was more highly expressed under control conditions in Maythorpe. The physiological consequences of altered vacuolar ATPase expression are discussed in relation to the salt sensitivity of Maythorpe.
dc.language.isoen
dc.publisherWiley InterScience
dc.relation.urlhttp://www3.interscience.wiley.com/journal/118970177/abstract?CRETRY=1&SRETRY=0
dc.subject.meshAdenosine Triphosphatases
dc.subject.meshBase Sequence
dc.subject.meshBlotting, Northern
dc.subject.meshBlotting, Southern
dc.subject.meshDNA
dc.subject.meshGene Expression Profiling
dc.subject.meshHordeum
dc.subject.meshMolecular Sequence Data
dc.subject.meshPlants
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshRNA, Messenger
dc.subject.meshSalts
dc.subject.meshSequence Analysis, DNA
dc.subject.meshVariation (Genetics)
dc.titleVariation between two near isogenic barley (Hordeum vulgare) cultivars in expression of the B subunit of the vacuolar ATPase in response to salinity.
dc.typeJournal article
dc.identifier.journalHereditas
html.description.abstractA gene encoding the barley vacuolar ATPase subunit B (BSVAP) was differentially expressed between two near isogenic barley cultivars, Golden Promise and Maythorpe. This gene (BSVAP) was isolated by the mRNA differential display technique (DDRT-PCR). BSVAP was salt inducible under long-term salinity stress in the salt sensitive cultivar Maythorpe but less so in the relatively salt tolerant Golden Promise and was more highly expressed under control conditions in Maythorpe. The physiological consequences of altered vacuolar ATPase expression are discussed in relation to the salt sensitivity of Maythorpe.


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