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dc.contributor.authorO'Neil, John D.
dc.contributor.authorBugno, Marcin
dc.contributor.authorStanley, Michele S.
dc.contributor.authorBarham-Morris, Julia B.
dc.contributor.authorWoodcock, Nicola A.
dc.contributor.authorClement, Darren J.
dc.contributor.authorClipson, Nicholas J. W.
dc.contributor.authorWhitehead, Michael P.
dc.contributor.authorFincham, Daron A.
dc.contributor.authorHooley, Paul
dc.date.accessioned2008-04-03T08:59:26Z
dc.date.available2008-04-03T08:59:26Z
dc.date.issued2002
dc.identifier.citationMycological Research, 106(4): 491-498
dc.identifier.issn09537562
dc.identifier.issn14698102
dc.identifier.doi10.1017/S0953756202005701
dc.identifier.urihttp://hdl.handle.net/2436/22192
dc.description.abstractWe report the cloning and sequencing of a DNA fragment encoding a putative C2H2 zinc finger protein from Aspergillus nidulans. The gene was isolated by complementation cloning of a salt sensitive phenotype of the A. nidulans sltAl mutant. A 3.8 kb Pst I fragment that restored wild type salt tolerance contained one large open reading frame of 2202 bp. The predicted protein (StzA) from this reading frame comprises 698 amino acids and has three Zinc fingers along with a putative transcriptional activation domain rich in acidic amino acids. The corresponding sequence from a sltAl mutant contains a premature STOP codon resulting in loss of the putative transcriptional activator in the C-terminal region. The Zinc fingers show conserved motifs with a number of transcription factors including CreA from A. nidulans and the human Wilm's tumour susceptibility protein WT-1.
dc.language.isoen
dc.publisherElsevier Science Direct
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7XMR-4RR97B2-J&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=af34e34456d8e3a7f9b82176773566fd
dc.subjectC2H2
dc.subjectZinc fingers
dc.subjectAspergillus nidulans
dc.subjectProteins
dc.subjectAmino Acids
dc.subjectAbiotic stress
dc.titleCloning of a novel gene encoding a C2H2 zinc finger protein that alleviates sensitivity to abiotic stresses in Aspergillus nidulans
dc.typeJournal article
dc.identifier.journalMycological Research
html.description.abstractWe report the cloning and sequencing of a DNA fragment encoding a putative C2H2 zinc finger protein from Aspergillus nidulans. The gene was isolated by complementation cloning of a salt sensitive phenotype of the A. nidulans sltAl mutant. A 3.8 kb Pst I fragment that restored wild type salt tolerance contained one large open reading frame of 2202 bp. The predicted protein (StzA) from this reading frame comprises 698 amino acids and has three Zinc fingers along with a putative transcriptional activation domain rich in acidic amino acids. The corresponding sequence from a sltAl mutant contains a premature STOP codon resulting in loss of the putative transcriptional activator in the C-terminal region. The Zinc fingers show conserved motifs with a number of transcription factors including CreA from A. nidulans and the human Wilm's tumour susceptibility protein WT-1.


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