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dc.contributor.authorChen, Wen
dc.contributor.authorStoddard, Fred L.
dc.contributor.authorBaldwin, Timothy C.
dc.date.accessioned2008-01-29T14:55:33Z
dc.date.available2008-01-29T14:55:33Z
dc.date.issued2006
dc.identifier.citationInternational Journal of Plant Sciences, 167(5): 919-932
dc.identifier.issn10585893
dc.identifier.issn15375315
dc.identifier.doi10.1086/506330
dc.identifier.urihttp://hdl.handle.net/2436/17033
dc.descriptionMetadata only
dc.description.abstractCell walls of the stigma and style are important zones for cell‐cell recognition, nutrition, guidance, and protection of the pollen tube along the transmitting tract. The objective of this study was to investigate the modulation of selected cell wall epitopes during pistil development in the crop species Vicia faba L. (faba bean). An immunocytochemical investigation of pistil development was performed using a panel of four anti–cell wall/plasma membrane antibodies at both light and electron microscope levels during the 4 days leading up to anthesis. The selected antibodies recognized mannan‐containing polysaccharides (antimannan antisera), β‐1,4‐galactan (LM5), α‐1,5‐l‐arabinan (LM6), and arabinogalactan/arabinogalactan‐protein (AG/AGP) epitopes, with GlcpUA‐β‐1,→3‐d‐GalpUA‐α‐1,→2‐l‐Rha trisaccharide motifs (JIM13). Mannan accumulation was shown to be associated with the formation and maturation of sclerenchyma cells and xylem elements. The β‐1,4‐galactan and α‐1,5‐l‐arabinan epitopes were highly expressed in the walls of parenchyma and epidermis in a developmentally regulated manner. AG/AGP epitopes were particularly abundant in the stigma and cells lining the stylar canal, together with the α‐1,5‐l‐arabinan epitope recognized by LM6. These data suggest an association of AGs/AGPs with pollen tube growth, of mannan with strengthening and stiffening the pistil, and of β‐1,4‐galactan and α‐1,5‐l‐arabinan components with tissue flexibility within the pistil.
dc.language.isoen
dc.publisherUniversity of Chicago Press
dc.relation.urlhttp://www.journals.uchicago.edu/doi/abs/10.1086/506330
dc.subjectFaba bean
dc.subjectOpen style
dc.subjectArabinogalactan-protein
dc.subjectβ‐galactan
dc.subjectMannan
dc.subjectα‐arabinan
dc.titleDevelopmental regulation of mannan, arabinogalactan-protein and pectic epitopes in pistils of Vicia faba (faba bean)
dc.typeJournal article
html.description.abstractCell walls of the stigma and style are important zones for cell‐cell recognition, nutrition, guidance, and protection of the pollen tube along the transmitting tract. The objective of this study was to investigate the modulation of selected cell wall epitopes during pistil development in the crop species Vicia faba L. (faba bean). An immunocytochemical investigation of pistil development was performed using a panel of four anti–cell wall/plasma membrane antibodies at both light and electron microscope levels during the 4 days leading up to anthesis. The selected antibodies recognized mannan‐containing polysaccharides (antimannan antisera), β‐1,4‐galactan (LM5), α‐1,5‐l‐arabinan (LM6), and arabinogalactan/arabinogalactan‐protein (AG/AGP) epitopes, with GlcpUA‐β‐1,→3‐d‐GalpUA‐α‐1,→2‐l‐Rha trisaccharide motifs (JIM13). Mannan accumulation was shown to be associated with the formation and maturation of sclerenchyma cells and xylem elements. The β‐1,4‐galactan and α‐1,5‐l‐arabinan epitopes were highly expressed in the walls of parenchyma and epidermis in a developmentally regulated manner. AG/AGP epitopes were particularly abundant in the stigma and cells lining the stylar canal, together with the α‐1,5‐l‐arabinan epitope recognized by LM6. These data suggest an association of AGs/AGPs with pollen tube growth, of mannan with strengthening and stiffening the pistil, and of β‐1,4‐galactan and α‐1,5‐l‐arabinan components with tissue flexibility within the pistil.


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