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    Identification of the pocket factors in a picornavirus

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    Authors
    Smyth, M.S.
    Martin, Jan H.
    Pettitt, T.
    Symonds, A.
    Issue Date
    2003
    
    Metadata
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    Abstract
    Bovine enterovirus (BEV), along with other enteroviruses and the rhinoviruses, has a hydrophobic pocket within structural protein VP1. In the crystal structures of these viruses there is electron density commensurate with a non-protein molecule within the pocket. These molecules, termed pocket factors, have been shown to stabilise the capsid and their removal from the pocket is a necessary prerequisite to uncoating. The pocket factors have been proposed, from the electron densities and uncoating studies, to be short chain fatty acids. In order to identify the pocket factor of BEV, we have grown and purified the virus in an identical manner to that used for the crystal structure determination and have performed a lipophilic extraction. Palmitic acid, C16:0, was the most abundant accounting for 40.8% by mass of the lipophilic extract (39.3 mol%). Myristic acid C14:0, was next most abundant at 18.5% by mass (20.0 mol%). In addition, we have identified other fatty acids in smaller proportions. We have therefore shown that BEV contains saturated fatty acid pocket factors of varying chain length. We have also compared the profile of the fatty acyl chain composition of BEV with those for uninfected BHK-21 cell plasma membrane and endoplasmic reticulum extracts.
    Citation
    Archives of Virology, 148(6): 1225-1233
    Publisher
    Springer Wien
    URI
    http://hdl.handle.net/2436/15892
    DOI
    10.1007/s00705-002-0974-4
    Additional Links
    http://direct.bl.uk/bld/PlaceOrder.do?UIN=132652958&ETOC=RN&from=searchenginehttp://www.springerlink.com/content/1432-8798/?k=identification+of+the+pocket+factors
    Type
    Journal article
    Language
    en
    Description
    Metadata only
    ISSN
    03048608,14328798
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00705-002-0974-4
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    Research Institute in Healthcare Science

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