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A novel multiplex RT-PCR system detects human endogenous retrovirus-K in breast cancer

dc.contributor.authorEjtehadi, H. Davari
dc.contributor.authorMartin, Jan H.
dc.contributor.authorJunying, J
dc.contributor.authorRoden, Denise A.
dc.contributor.authorLahiri, M.
dc.contributor.authorWarren, Phil
dc.contributor.authorMurray, Paul G.
dc.contributor.authorNelson, Paul N.
dc.date.accessioned2008-01-09T14:26:32Z
dc.date.available2008-01-09T14:26:32Z
dc.date.issued2005
dc.identifier.citationArchives of Virology, 150(1): 177-184
dc.identifier.issn03048608,14328798
dc.identifier.doi10.1007/s00705-004-0378-8
dc.identifier.urihttp://hdl.handle.net/2436/15870
dc.descriptionMetadata only.
dc.description.abstractHuman endogenous retrovirus HERV-K like-sequences have been implicated in certain cancers. We developed a novel multiplex RT-PCR system for HERV-K that yielded a 533bp product together with a smaller sized product (319bp) of the house keeping gene, histidyl tRNA synthetase (HtRNAS). The latter spanned an intron that also served to validate target cDNA. PCR amplicons of HERV-K and HtRNAS were visualised using a gel documentation system and the pixel intensity used to derive semi-quantitative levels of viral expression. Our data showed that HERV-K10 was significantly elevated in MCF-7 cells treated with estrogen. Interestingly, HERV-K expression was higher in MCF-7 cells selected with adriamycin. RT-PCR combined with Southern blotting also detected HERV-K from breast cancer tissue using laser capture microscopy. This study highlights the presence of HERV-K in the breast cancer cell lines MCF-7 and MCF-7 ADR and confirms HERV-K10 transcripts in the cell line T47D. We believe this study to be a novel approach in determining levels of HERV-K expression and for detecting this virus in cancer cell lines and tissues.
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.urlhttp://www.springerlink.com/content/1432-8798/?k=a+novel+multiplex+RT-PCR
dc.subjectHuman Endogenous Retroviruses
dc.subjectRT-PCR system
dc.subjectBiomedical science
dc.subjectMedicine
dc.subjectLife science
dc.titleA novel multiplex RT-PCR system detects human endogenous retrovirus-K in breast cancer
dc.typeJournal article
html.description.abstractHuman endogenous retrovirus HERV-K like-sequences have been implicated in certain cancers. We developed a novel multiplex RT-PCR system for HERV-K that yielded a 533bp product together with a smaller sized product (319bp) of the house keeping gene, histidyl tRNA synthetase (HtRNAS). The latter spanned an intron that also served to validate target cDNA. PCR amplicons of HERV-K and HtRNAS were visualised using a gel documentation system and the pixel intensity used to derive semi-quantitative levels of viral expression. Our data showed that HERV-K10 was significantly elevated in MCF-7 cells treated with estrogen. Interestingly, HERV-K expression was higher in MCF-7 cells selected with adriamycin. RT-PCR combined with Southern blotting also detected HERV-K from breast cancer tissue using laser capture microscopy. This study highlights the presence of HERV-K in the breast cancer cell lines MCF-7 and MCF-7 ADR and confirms HERV-K10 transcripts in the cell line T47D. We believe this study to be a novel approach in determining levels of HERV-K expression and for detecting this virus in cancer cell lines and tissues.


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