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    A novel multiplex RT-PCR system detects human endogenous retrovirus-K in breast cancer

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    Authors
    Ejtehadi, H. Davari
    Martin, Jan H.
    Junying, J
    Roden, Denise A.
    Lahiri, M.
    Warren, Phil
    Murray, Paul G.
    Nelson, Paul N.
    Issue Date
    2005
    
    Metadata
    Show full item record
    Abstract
    Human endogenous retrovirus HERV-K like-sequences have been implicated in certain cancers. We developed a novel multiplex RT-PCR system for HERV-K that yielded a 533bp product together with a smaller sized product (319bp) of the house keeping gene, histidyl tRNA synthetase (HtRNAS). The latter spanned an intron that also served to validate target cDNA. PCR amplicons of HERV-K and HtRNAS were visualised using a gel documentation system and the pixel intensity used to derive semi-quantitative levels of viral expression. Our data showed that HERV-K10 was significantly elevated in MCF-7 cells treated with estrogen. Interestingly, HERV-K expression was higher in MCF-7 cells selected with adriamycin. RT-PCR combined with Southern blotting also detected HERV-K from breast cancer tissue using laser capture microscopy. This study highlights the presence of HERV-K in the breast cancer cell lines MCF-7 and MCF-7 ADR and confirms HERV-K10 transcripts in the cell line T47D. We believe this study to be a novel approach in determining levels of HERV-K expression and for detecting this virus in cancer cell lines and tissues.
    Citation
    Archives of Virology, 150(1): 177-184
    Publisher
    Springer Wien
    URI
    http://hdl.handle.net/2436/15870
    DOI
    10.1007/s00705-004-0378-8
    Additional Links
    http://www.springerlink.com/content/1432-8798/?k=a+novel+multiplex+RT-PCR
    Type
    Journal article
    Language
    en
    Description
    Metadata only.
    ISSN
    03048608,14328798
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00705-004-0378-8
    Scopus Count
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    Research Institute in Healthcare Science

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