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dc.contributor.authorYue, Patrick Y. K.
dc.contributor.authorWong, Daisy Y. L.
dc.contributor.authorHa, Wai-Yan
dc.contributor.authorFung, M.C.
dc.contributor.authorMak, Nai Ki
dc.contributor.authorYeung, H.W.
dc.contributor.authorLeung, Hei Wun
dc.contributor.authorChan, Kelvin C.
dc.contributor.authorLiu, Liang
dc.contributor.authorFan, T. P. David
dc.contributor.authorWong, Ricky N. S.
dc.date.accessioned2008-01-08T15:08:35Z
dc.date.available2008-01-08T15:08:35Z
dc.date.issued2005
dc.identifier.citationAngiogenesis, 8(3): 205-216
dc.identifier.issn0969-6970
dc.identifier.pmid16328162
dc.identifier.doi10.1007/s10456-005-9000-2
dc.identifier.urihttp://hdl.handle.net/2436/15833
dc.descriptionMetadata only
dc.description.abstractThe major active constituents of ginseng are ginsenosides, and Rg(1) is a predominant compound of the total extract. Recent studies have demonstrated that Rg(1) can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg(1). We report that Rg(1) induces the proliferation of HUVECs, monitored using [(3)H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg(1) (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg(1) was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg(1) revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg(1) can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.
dc.language.isoen
dc.publisherSpringer Verlag
dc.relation.urlhttp://www.springerlink.com/content/y77908j6248l6362/
dc.subjectAngiogenesis
dc.subjectGene Expression Profiling
dc.subjectGinsenosides
dc.subjectHUVEC
dc.subjectMicroarray
dc.subjectRg1
dc.titleElucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro.
dc.typeJournal article
html.description.abstractThe major active constituents of ginseng are ginsenosides, and Rg(1) is a predominant compound of the total extract. Recent studies have demonstrated that Rg(1) can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg(1). We report that Rg(1) induces the proliferation of HUVECs, monitored using [(3)H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg(1) (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg(1) was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg(1) revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg(1) can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.


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