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    The validity of capillary blood sampling in the determinationof human growth hormone concentration during exercise in men

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    Authors
    Godfrey, R.J.
    Whyte, Gregory P.
    McCarthy, J.
    Nevill, Alan M.
    Head, Andrew
    Issue Date
    2004
    Submitted date
    2007-12
    
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    Abstract
    Background: Studies measuring human growth hormone (hGH) in blood during exercise have mainly used venous sampling. The invasive nature of this procedure makes evaluation of hGH impossible in various exercise environments. Objective: To determine whether capillary sampling could offer an alternative sampling method. Methods: Capillary and venous blood samples were collected for determination of hGH at the end of each exercise stage during an incremental exercise test in 16 male club level competitive cyclists (mean (SD) age 30.8 (8.0) years, body mass 72.2 (7.1) kg, body fat 12.9 (3.5)%, peak oxygen consumption 4.18 (0.46) l?min21). Linear regression, from a plot of venous v capillary blood hGH concentration, showed a correlation coefficient of r = 0.986 (p,0.001). When geometric means and log transformations were used, a coefficient of variation of 14.2% was demonstrated between venous and capillary flow for hGH concentration. The mean ratio limits of agreement were 0.62 (1.72)—that is, 95% of the ratios were contained between 0.36 and 1.07, with a mean of 0.62. Conclusions: Capillary blood sampling is an acceptable alternative to venous sampling for determining hGH concentration during rest and exercise. Sample sites should not be used interchangeably: one site should be chosen and its use standardised.
    Citation
    British Journal of Sports Medicine, 38 (e27)
    Publisher
    BMJ publishing
    URI
    http://hdl.handle.net/2436/14993
    DOI
    10.1136/bjsm.2003.008714
    Additional Links
    http://bjsm.bmj.com/cgi/content/abstract/38/5/e27
    Type
    Journal article
    Language
    en
    ISSN
    03063674
    ae974a485f413a2113503eed53cd6c53
    10.1136/bjsm.2003.008714
    Scopus Count
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    Faculty of Education, Health and Wellbeing

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