Show simple item record

dc.contributor.authorKnigge, Thomas
dc.contributor.authorMann, Neelam
dc.contributor.authorParveen, Zahida
dc.contributor.authorPerry, Christopher
dc.contributor.authorGernhöfer, Maike
dc.contributor.authorTriebskorn, Rita
dc.contributor.authorKöhler, Heinz-R
dc.contributor.authorConnock, Martin
dc.date.accessioned2007-11-19T12:42:11Z
dc.date.available2007-11-19T12:42:11Z
dc.date.issued2002
dc.identifier.citationComparative Biochemistry and Physiology Part C Toxicology & Pharmacology 2002, 131(3): 259-269
dc.identifier.issn15320456
dc.identifier.doi10.1016/S1532-0456(02)00013-3
dc.identifier.urihttp://hdl.handle.net/2436/14649
dc.descriptionMetadadata only
dc.description.abstractAmongst animals, several hydrogen peroxide-generating oxidases are apparently restricted to molluscs. One of these, -mannitol oxidase, is concentrated in the alimentary system, where it is associated with its own subcellular membrane system of unique tubular morphology, most likely representing a structural modification of the ER. These structures can be purified by subcellular fractionation and have been termed ‘mannosomes’. Little is known about the functions of mannitol oxidase or of mannosomes, but the previously reported molluscicide-induced increase in mannosomes implies their involvement in a general stress reaction. In this study, we examined the effects of heavy metal stress in the terrestrial gastropod Arion lusitanicus. The activity of mannitol oxidase and mannosome abundance were monitored, together with metal effects on heat-shock protein level, and these parameters were compared to heavy metal accumulation in the digestive gland. We found that mannitol oxidase is inhibited by heavy metals more than other oxidases. On the other hand, hsp70 levels and mannosomal protein were increased with enhanced heavy metal stress, the latter indicating a probable increase in the number of mannosome organelles. Thus, stress protein (hsp70) and mannosomal protein were positively correlated with heavy metal accumulation, whereas the enzyme activity showed a negative correlation with increasing heavy metal content of the slugs.
dc.language.isoen
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W89-452F7N2-3&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=562af2dc8f70808ea43c318ede896f8b
dc.subjectArion lusitanicus
dc.subjectSlug
dc.subjectDigestive gland
dc.subjectGenerating oxidases
dc.subjectEnzyme
dc.subjectGastropoda
dc.subjectHeat-shock protein (hsp70)
dc.subjectEpatopancreas
dc.subjectOrganelle
dc.subjectH2O2-generating oxidases
dc.titleMannosomes: a molluscan intracellular tubular membrane system related to heavy metal stress?
dc.typeJournal article
dc.format.digYES
html.description.abstractAmongst animals, several hydrogen peroxide-generating oxidases are apparently restricted to molluscs. One of these, -mannitol oxidase, is concentrated in the alimentary system, where it is associated with its own subcellular membrane system of unique tubular morphology, most likely representing a structural modification of the ER. These structures can be purified by subcellular fractionation and have been termed ‘mannosomes’. Little is known about the functions of mannitol oxidase or of mannosomes, but the previously reported molluscicide-induced increase in mannosomes implies their involvement in a general stress reaction. In this study, we examined the effects of heavy metal stress in the terrestrial gastropod Arion lusitanicus. The activity of mannitol oxidase and mannosome abundance were monitored, together with metal effects on heat-shock protein level, and these parameters were compared to heavy metal accumulation in the digestive gland. We found that mannitol oxidase is inhibited by heavy metals more than other oxidases. On the other hand, hsp70 levels and mannosomal protein were increased with enhanced heavy metal stress, the latter indicating a probable increase in the number of mannosome organelles. Thus, stress protein (hsp70) and mannosomal protein were positively correlated with heavy metal accumulation, whereas the enzyme activity showed a negative correlation with increasing heavy metal content of the slugs.


Files in this item

Thumbnail
Name:
Publisher version

This item appears in the following Collection(s)

Show simple item record