Localisation and characterisation of cell wall mannan polysaccharides in Arabidopsis thaliana
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsHandford, Michael G.
Baldwin, Timothy C.
Prime, Tracy A.
MetadataShow full item record
AbstractPolysaccharides containing b-1,4-mannosyl residues (mannans) are abundant in the lignified secondary cell walls of gymnosperms, and are also found as major seed storage polysaccharides in some plants, such as legume species. Although they have been found in a variety of angiosperm tissues, little is known about their presence and tissue localisation in the model angiosperm, Arabidopsis thaliana (L.) Heynh. In this study, antibodies that specifically recognised mannans in competitive ELISA experiments were raised in rabbits. Using these antibodies, we showed that Golgi-rich vesicles derived from Arabidopsis callus were able to synthesise mannan polysaccharides in vitro. Immunofluorescence light microscopy and immunogold electron microscopy of Arabidopsis inflorescence stem sections revealed that the mannan polysaccharide epitopes were localised in the thickened secondary cell walls of xylem elements, xylem parenchyma and interfascicular fibres. Similarly, mannan epitopes were present in the xylem of the leaf vascular bundles. Surprisingly, the thickened epidermal cell walls of both leaves and stems also contained abundant mannan epitopes. Low levels were observed in most other cell types examined. Thus, mannans are widespread in Arabidopsis tissues, may be of particular significance in both lignified and non-lignified thickened cell walls. Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) of cell wall preparations digested with a specific mannanase showed that there is glucomannan in inflorescence stems. The findings show that Arabidopsis can be used as a model plant in studies of the synthesis and functions of mannans.
CitationPlanta 2003, 218(1): 27-36
CollectionsAgriculture Research Group
- Cell wall polysaccharides from fern leaves: evidence for a mannan-rich Type III cell wall in Adiantum raddianum.
- Authors: Silva GB, Ionashiro M, Carrara TB, Crivellari AC, Tiné MA, Prado J, Carpita NC, Buckeridge MS
- Issue date: 2011 Dec
- Functional genomic analysis supports conservation of function among cellulose synthase-like a gene family members and suggests diverse roles of mannans in plants.
- Authors: Liepman AH, Nairn CJ, Willats WG, Sørensen I, Roberts AW, Keegstra K
- Issue date: 2007 Apr
- Enzymatic fingerprinting of Arabidopsis pectic polysaccharides using polysaccharide analysis by carbohydrate gel electrophoresis (PACE).
- Authors: Barton CJ, Tailford LE, Welchman H, Zhang Z, Gilbert HJ, Dupree P, Goubet F
- Issue date: 2006 Jun
- Mannan transglycosylase: a novel enzyme activity in cell walls of higher plants.
- Authors: Schröder R, Wegrzyn TF, Bolitho KM, Redgwell RJ
- Issue date: 2004 Aug