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dc.contributor.authorZhang, Haiyu
dc.contributor.authorGómez-García, Maria R.
dc.contributor.authorBrown, Michael R. W.
dc.contributor.authorKornberg, Arthur
dc.date.accessioned2007-04-05T11:34:44Z
dc.date.available2007-04-05T11:34:44Z
dc.date.issued2005
dc.date.submitted2007-01-26
dc.identifier.citationProc. Natl. Acad. Sci. U.S.A. 2005, 102(8):2731-5
dc.identifier.issn0027-8424
dc.identifier.pmid15701689
dc.identifier.doi10.1073/pnas.0500023102
dc.identifier.urihttp://hdl.handle.net/2436/11143
dc.description.abstractDictyostelium discoideum, a social slime mold that forms fruiting bodies with spores, depends on inorganic polyphosphate (poly P) for its cycles of development and for nutritional predation on bacteria. The synthesis of poly P, a polymer of tens or hundreds of phosphate residues linked by high energy, ATP-like bonds, is catalyzed in most bacteria by poly P kinase (PPK1). The eukaryote D. discoideum possesses a homolog of PPK1. We report here that mutants of D. discoideum PPK1 (DdPPK1) have reduced levels of poly P and are deficient in development. Fruiting bodies are smaller and produce fewer spores, which appear to germinate like the wild type (WT). The DdPPK1 mutant formed smaller plaques on bacterial lawns compared with those of the WT. Predation by D. discoideum, assessed by uptake and digestion of Klebsiella aerogenes, showed that fewer bacteria were taken up by the DdPPK1 mutant compared with the WT and were killed less rapidly, indicating a role of poly P and/or DdPPK1 in phagocytosis. On Pseudomonas aeruginosa lawns, cleared plaques were observed with the bacterial PPK1 mutant but not with the WT P. aeruginosa. Thus, poly P is important in predation both for the predator and prey.
dc.format.extent351694 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.relation.urlhttp://www.pnas.org/content/102/8/2731
dc.subjectDictyostelium discoideum
dc.subjectInorganic polyphosphate
dc.subjectSporulation
dc.subjectPredation
dc.titleInorganic polyphosphate in Dictyostelium discoideum: influence on development, sporulation, and predation.
dc.typeJournal article
dc.format.digYES
refterms.dateFOA2018-08-21T09:42:03Z
html.description.abstractDictyostelium discoideum, a social slime mold that forms fruiting bodies with spores, depends on inorganic polyphosphate (poly P) for its cycles of development and for nutritional predation on bacteria. The synthesis of poly P, a polymer of tens or hundreds of phosphate residues linked by high energy, ATP-like bonds, is catalyzed in most bacteria by poly P kinase (PPK1). The eukaryote D. discoideum possesses a homolog of PPK1. We report here that mutants of D. discoideum PPK1 (DdPPK1) have reduced levels of poly P and are deficient in development. Fruiting bodies are smaller and produce fewer spores, which appear to germinate like the wild type (WT). The DdPPK1 mutant formed smaller plaques on bacterial lawns compared with those of the WT. Predation by D. discoideum, assessed by uptake and digestion of Klebsiella aerogenes, showed that fewer bacteria were taken up by the DdPPK1 mutant compared with the WT and were killed less rapidly, indicating a role of poly P and/or DdPPK1 in phagocytosis. On Pseudomonas aeruginosa lawns, cleared plaques were observed with the bacterial PPK1 mutant but not with the WT P. aeruginosa. Thus, poly P is important in predation both for the predator and prey.


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