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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Pharmacology Research Group > Critical value determination on similarity of fingerprints

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/9858
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Title: Critical value determination on similarity of fingerprints
Authors: Fang, Kai-Tai
Liang, Yi-Zeng
Yin, Xiao-lin
Chan, Kelvin C.
Lu, Guang-Hua
Citation: Chemometrics and Intelligent Laboratory Systems, 82(1-2): 236-240
Publisher: Elsevier
Issue Date: 2006
URI: http://hdl.handle.net/2436/9858
DOI: 10.1016/j.chemolab.2005.06.018
Additional Links: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFP-4H6PKC5-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=459fbcd411616624db24a8594c886284
Abstract: A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.
Type: Article
Language: en
Keywords: Angelica spp
Ligusticum chuanxiong
Cnidium officinale
Umbelliferae herbs
Chromatographic fingerprints
ISSN: 01697439
Appears in Collections: Molecular Pharmacology Research Group

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