2.50
Hdl Handle:
http://hdl.handle.net/2436/9858
Title:
Critical value determination on similarity of fingerprints
Authors:
Fang, Kai-Tai; Liang, Yi-Zeng; Yin, Xiao-lin; Chan, Kelvin C.; Lu, Guang-Hua
Abstract:
A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.
Citation:
Chemometrics and Intelligent Laboratory Systems, 82(1-2): 236-240
Publisher:
Elsevier
Issue Date:
2006
URI:
http://hdl.handle.net/2436/9858
DOI:
10.1016/j.chemolab.2005.06.018
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFP-4H6PKC5-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=459fbcd411616624db24a8594c886284
Type:
Article
Language:
en
ISSN:
01697439
Appears in Collections:
Molecular Pharmacology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorFang, Kai-Tai-
dc.contributor.authorLiang, Yi-Zeng-
dc.contributor.authorYin, Xiao-lin-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorLu, Guang-Hua-
dc.date.accessioned2007-03-07T15:35:16Z-
dc.date.available2007-03-07T15:35:16Z-
dc.date.issued2006-
dc.identifier.citationChemometrics and Intelligent Laboratory Systems, 82(1-2): 236-240en
dc.identifier.issn01697439-
dc.identifier.doi10.1016/j.chemolab.2005.06.018-
dc.identifier.urihttp://hdl.handle.net/2436/9858-
dc.description.abstractA high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.en
dc.format.extent405654 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFP-4H6PKC5-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=459fbcd411616624db24a8594c886284en
dc.subjectAngelica sppen
dc.subjectLigusticum chuanxiongen
dc.subjectCnidium officinaleen
dc.subjectUmbelliferae herbsen
dc.subjectChromatographic fingerprintsen
dc.titleCritical value determination on similarity of fingerprintsen
dc.typeArticleen
dc.format.digYES-
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