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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Pharmacology Research Group > The isolation, identification and determination of dehydrotumulosic acid in Poria cocos.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/9856
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Title: The isolation, identification and determination of dehydrotumulosic acid in Poria cocos.
Authors: Song, Zonghua
Bi, Kaishun
Luo, Xu
Chan, Kelvin C.
Citation: Analytical Sciences, 18(5): 529-531
Publisher: The Japan Society for Analytical Chemistry
Issue Date: 2002
URI: http://hdl.handle.net/2436/9856
DOI: 10.2116/analsci.18.529
PubMed ID: 12036119
Additional Links: http://www.jstage.jst.go.jp/article/analsci/18/5/18_529/_article
http://dx.doi.org/10.2116/analsci.18.529
Abstract: Poria cocos (Fuling), a popular Chinese medicinal (CM) herb of fungal origin, has been included in many combinations with other CM herbs for its traditionally claimed activities of inducing diuresis, excreting dampness, invigorating the spleen and tranquilizing the mind and its modern pharmacological use of modulating the immune system of the body. Dehydrotumulosic acid, one of the effective constituents of Fuling, was isolated from the chloroform-soluble material of ethanol extract of the fungus. After further purification by a high-performance liquid chromatographic method on a C18 column, the purified constituent was identified using modern analytical techniques, such as UV, 13C-NMR and EI-MS. A reversed-phase high-performance liquid chromatographic method has been developed for the determination of dehydrotumulosic acid in Poria cocos. The determination can be accomplished in less than 50 min using methanol-acetonitrile-2% glacial acetic acid as the mobile phase at a flow rate of 1.0 mL/min, with a UV detector setting at 242 nm and testosterone propionate used as an internal standard. This assay for dehydrotumulosic acid is simple, rapid and with good reproducibility.
Type: Article
Language: en
Keywords: Dehydrotumulosic acid
Poria cocos
Chinese medicine
Chinese herbs
Medicine, Chinese Traditional
ISSN: 0910-6340
Appears in Collections: Molecular Pharmacology Research Group

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