The isolation, identification and determination of dehydrotumulosic acid in Poria cocos.

2.50
Hdl Handle:
http://hdl.handle.net/2436/9856
Title:
The isolation, identification and determination of dehydrotumulosic acid in Poria cocos.
Authors:
Song, Zonghua; Bi, Kaishun; Luo, Xu; Chan, Kelvin C.
Abstract:
Poria cocos (Fuling), a popular Chinese medicinal (CM) herb of fungal origin, has been included in many combinations with other CM herbs for its traditionally claimed activities of inducing diuresis, excreting dampness, invigorating the spleen and tranquilizing the mind and its modern pharmacological use of modulating the immune system of the body. Dehydrotumulosic acid, one of the effective constituents of Fuling, was isolated from the chloroform-soluble material of ethanol extract of the fungus. After further purification by a high-performance liquid chromatographic method on a C18 column, the purified constituent was identified using modern analytical techniques, such as UV, 13C-NMR and EI-MS. A reversed-phase high-performance liquid chromatographic method has been developed for the determination of dehydrotumulosic acid in Poria cocos. The determination can be accomplished in less than 50 min using methanol-acetonitrile-2% glacial acetic acid as the mobile phase at a flow rate of 1.0 mL/min, with a UV detector setting at 242 nm and testosterone propionate used as an internal standard. This assay for dehydrotumulosic acid is simple, rapid and with good reproducibility.
Citation:
Analytical Sciences, 18(5): 529-531
Publisher:
The Japan Society for Analytical Chemistry
Issue Date:
2002
URI:
http://hdl.handle.net/2436/9856
DOI:
10.2116/analsci.18.529
PubMed ID:
12036119
Additional Links:
http://www.jstage.jst.go.jp/article/analsci/18/5/18_529/_article; http://dx.doi.org/10.2116/analsci.18.529
Submitted date:
2007-02-07
Type:
Article
Language:
en
ISSN:
0910-6340
Appears in Collections:
Molecular Pharmacology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSong, Zonghua-
dc.contributor.authorBi, Kaishun-
dc.contributor.authorLuo, Xu-
dc.contributor.authorChan, Kelvin C.-
dc.date.accessioned2007-03-07T15:22:55Z-
dc.date.available2007-03-07T15:22:55Z-
dc.date.issued2002-
dc.date.submitted2007-02-07-
dc.identifier.citationAnalytical Sciences, 18(5): 529-531en
dc.identifier.issn0910-6340-
dc.identifier.pmid12036119-
dc.identifier.doi10.2116/analsci.18.529-
dc.identifier.urihttp://hdl.handle.net/2436/9856-
dc.description.abstractPoria cocos (Fuling), a popular Chinese medicinal (CM) herb of fungal origin, has been included in many combinations with other CM herbs for its traditionally claimed activities of inducing diuresis, excreting dampness, invigorating the spleen and tranquilizing the mind and its modern pharmacological use of modulating the immune system of the body. Dehydrotumulosic acid, one of the effective constituents of Fuling, was isolated from the chloroform-soluble material of ethanol extract of the fungus. After further purification by a high-performance liquid chromatographic method on a C18 column, the purified constituent was identified using modern analytical techniques, such as UV, 13C-NMR and EI-MS. A reversed-phase high-performance liquid chromatographic method has been developed for the determination of dehydrotumulosic acid in Poria cocos. The determination can be accomplished in less than 50 min using methanol-acetonitrile-2% glacial acetic acid as the mobile phase at a flow rate of 1.0 mL/min, with a UV detector setting at 242 nm and testosterone propionate used as an internal standard. This assay for dehydrotumulosic acid is simple, rapid and with good reproducibility.en
dc.format.extent39911 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherThe Japan Society for Analytical Chemistryen
dc.relation.urlhttp://www.jstage.jst.go.jp/article/analsci/18/5/18_529/_articleen
dc.relation.urlhttp://dx.doi.org/10.2116/analsci.18.529en
dc.subjectDehydrotumulosic aciden
dc.subjectPoria cocosen
dc.subjectChinese medicineen
dc.subjectChinese herbsen
dc.subjectMedicine, Chinese Traditional-
dc.titleThe isolation, identification and determination of dehydrotumulosic acid in Poria cocos.en
dc.typeArticleen
dc.format.digYES-
All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.