Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs.

2.50
Hdl Handle:
http://hdl.handle.net/2436/9849
Title:
Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs.
Authors:
Lu, Guang-Hua; Chan, Kelvin C.; Liang, Yi-Zeng; Leung, Kelvin Sze-Yin; Chan, Chi-Leung; Jiang, Zhi-Hong; Zhao, Zhong-Zhen
Abstract:
A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.
Citation:
Journal of Chromatography A, 1073(1-2): 383-392
Publisher:
Elsevier
Issue Date:
2005
URI:
http://hdl.handle.net/2436/9849
DOI:
10.1016/j.chroma.2004.11.080
PubMed ID:
15909545
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4F1GYXY-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=3309f2e3b34844ab2c0881de1cb6f789
Submitted date:
2007-02-07
Type:
Article
Language:
en
ISSN:
0021-9673
Appears in Collections:
Molecular Pharmacology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Guang-Hua-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorLiang, Yi-Zeng-
dc.contributor.authorLeung, Kelvin Sze-Yin-
dc.contributor.authorChan, Chi-Leung-
dc.contributor.authorJiang, Zhi-Hong-
dc.contributor.authorZhao, Zhong-Zhen-
dc.date.accessioned2007-03-07T14:50:34Z-
dc.date.available2007-03-07T14:50:34Z-
dc.date.issued2005-
dc.date.submitted2007-02-07-
dc.identifier.citationJournal of Chromatography A, 1073(1-2): 383-392en
dc.identifier.issn0021-9673-
dc.identifier.pmid15909545-
dc.identifier.doi10.1016/j.chroma.2004.11.080-
dc.identifier.urihttp://hdl.handle.net/2436/9849-
dc.description.abstractA high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.en
dc.format.extent359273 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4F1GYXY-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=3309f2e3b34844ab2c0881de1cb6f789en
dc.subjectAngelica sppen
dc.subjectLigusticum chuanxiongen
dc.subjectCnidium officinaleen
dc.subjectUmbelliferae herbsen
dc.subjectChromatographic fingerprintsen
dc.titleDevelopment of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs.en
dc.typeArticleen
dc.format.digYES-
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