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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Pharmacology Research Group > Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/9849
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Title: Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs.
Authors: Lu, Guang-Hua
Chan, Kelvin C.
Liang, Yi-Zeng
Leung, Kelvin Sze-Yin
Chan, Chi-Leung
Jiang, Zhi-Hong
Zhao, Zhong-Zhen
Citation: Journal of Chromatography A, 1073(1-2): 383-392
Publisher: Elsevier
Issue Date: 2005
URI: http://hdl.handle.net/2436/9849
DOI: 10.1016/j.chroma.2004.11.080
PubMed ID: 15909545
Additional Links: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4F1GYXY-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=3309f2e3b34844ab2c0881de1cb6f789
Abstract: A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.
Type: Article
Language: en
Keywords: Angelica spp
Ligusticum chuanxiong
Cnidium officinale
Umbelliferae herbs
Chromatographic fingerprints
ISSN: 0021-9673
Appears in Collections: Molecular Pharmacology Research Group

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