Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells.

2.50
Hdl Handle:
http://hdl.handle.net/2436/9833
Title:
Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells.
Authors:
Leung, Kar Wah; Cheng, Yuen-Kit; Mak, Nai Ki; Chan, Kelvin C.; Fan, T. P. David; Wong, Ricky N. S.
Abstract:
We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.
Citation:
FEBS Letters, 580(13): 3211-3216
Publisher:
Elsevier
Journal:
FEBS Letters
Issue Date:
2006
URI:
http://hdl.handle.net/2436/9833
DOI:
10.1016/j.febslet.2006.04.080
PubMed ID:
16696977
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0014579306005370
Submitted date:
2007-02-01
Type:
Article
Language:
en
ISSN:
0014-5793
Appears in Collections:
Molecular Pharmacology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorLeung, Kar Wah-
dc.contributor.authorCheng, Yuen-Kit-
dc.contributor.authorMak, Nai Ki-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorFan, T. P. David-
dc.contributor.authorWong, Ricky N. S.-
dc.date.accessioned2007-03-07T13:53:05Z-
dc.date.available2007-03-07T13:53:05Z-
dc.date.issued2006-
dc.date.submitted2007-02-01-
dc.identifier.citationFEBS Letters, 580(13): 3211-3216en
dc.identifier.issn0014-5793-
dc.identifier.pmid16696977-
dc.identifier.doi10.1016/j.febslet.2006.04.080-
dc.identifier.urihttp://hdl.handle.net/2436/9833-
dc.description.abstractWe here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.en
dc.format.extent395944 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0014579306005370en
dc.subjectGinsenoside-Rg1en
dc.subjectNitric aciden
dc.subjectEndothelial cellsen
dc.titleSignaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells.en
dc.typeArticleen
dc.identifier.journalFEBS Letters-
dc.format.digYES-
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