Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells.
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Abstract
We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.Citation
FEBS Letters, 580(13): 3211-3216Publisher
ElsevierJournal
FEBS LettersPubMed ID
16696977Additional Links
http://linkinghub.elsevier.com/retrieve/pii/S0014579306005370Type
Journal articleLanguage
enISSN
0014-5793ae974a485f413a2113503eed53cd6c53
10.1016/j.febslet.2006.04.080
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