Characterisation of epitopes of pan-IgG/anti-G3m(u) and anti-Fc monoclonal antibodies.

2.50
Hdl Handle:
http://hdl.handle.net/2436/7751
Title:
Characterisation of epitopes of pan-IgG/anti-G3m(u) and anti-Fc monoclonal antibodies.
Authors:
Nelson, Paul N.; Westwood, Olwyn M. R.; Soltys, Andy; Jefferis, Roy; Goodall, Margaret; Baumforth, Karl R. N.; Frampton, Geoffrey; Tribbick, Gordon; Roden, Denise A.; Hay, Frank C.
Abstract:
The characterisation of monoclonal antibodies (MAbs) and their epitopes is important prior to their application as molecular probes. In this study, Western blotting using IgG1 Fc and pFc' fragments was employed to screen seven MAbs before pepscan analysis to determine their reactivity to potentially linear epitopes. MAbs PNF69C, PNF110A, X1A11 and MAbs WC2, G7C, JD312, 1A1 detected epitopes within the C(H)3 and C(H)2 domains, respectively. However, only four MAbs showed pepscan profiles that highlighted likely target residues. In particular, MAbs PNF69C and PNF110A that have previously been characterised with pan-IgG and anti-G3m(u) specificity, detected the peptide motif 338-KAKGQPR-344 which was located within the N-terminal region of the C(H)3 domain. Furthermore the majority of residues were present in all four IgG subclasses. Consequently the peptide identified was consistent with the pan-IgG nature of these antibodies. By using PCImdad, a molecular display programme, this sequence was visualised as surface accessible, located in the C(H)2/C(H)3 inter-domain region and proximal to the residue arginine(435). It is speculated that this residue may be important for phenotypic expression of G3m(u) and specificity of these reagents. Pepscan analysis of MAbs G7C and JD312 (both pan-IgG) highlighted the core peptide sequence 290-KPREE-294, which was present in the C(H)2 domain and was common to all four IgG subclasses. PCImdad also showed this region to be highly accessible and was consistent with previous bioinformatic and autoimmune analysis of IgG. Overall these MAbs may serve as useful anti-IgG or anti-G3m(u) reagents and probes of immunoglobulin structure.
Citation:
Immunology Letters, 88(1): 77-83
Publisher:
Elsevier BV
Issue Date:
2003
URI:
http://hdl.handle.net/2436/7751
DOI:
10.1016/S0165-2478(03)00056-7
PubMed ID:
12853166
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T75-48BM4JT-4&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=5c4e2c50f2e7c01a63119cee94260d4d
Submitted date:
2007-01-24
Type:
Article
Language:
en
ISSN:
0165-2478
Appears in Collections:
Molecular Immunology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorNelson, Paul N.-
dc.contributor.authorWestwood, Olwyn M. R.-
dc.contributor.authorSoltys, Andy-
dc.contributor.authorJefferis, Roy-
dc.contributor.authorGoodall, Margaret-
dc.contributor.authorBaumforth, Karl R. N.-
dc.contributor.authorFrampton, Geoffrey-
dc.contributor.authorTribbick, Gordon-
dc.contributor.authorRoden, Denise A.-
dc.contributor.authorHay, Frank C.-
dc.date.accessioned2007-01-24T16:57:09Z-
dc.date.available2007-01-24T16:57:09Z-
dc.date.issued2003-
dc.date.submitted2007-01-24-
dc.identifier.citationImmunology Letters, 88(1): 77-83en
dc.identifier.issn0165-2478-
dc.identifier.pmid12853166-
dc.identifier.doi10.1016/S0165-2478(03)00056-7-
dc.identifier.urihttp://hdl.handle.net/2436/7751-
dc.description.abstractThe characterisation of monoclonal antibodies (MAbs) and their epitopes is important prior to their application as molecular probes. In this study, Western blotting using IgG1 Fc and pFc' fragments was employed to screen seven MAbs before pepscan analysis to determine their reactivity to potentially linear epitopes. MAbs PNF69C, PNF110A, X1A11 and MAbs WC2, G7C, JD312, 1A1 detected epitopes within the C(H)3 and C(H)2 domains, respectively. However, only four MAbs showed pepscan profiles that highlighted likely target residues. In particular, MAbs PNF69C and PNF110A that have previously been characterised with pan-IgG and anti-G3m(u) specificity, detected the peptide motif 338-KAKGQPR-344 which was located within the N-terminal region of the C(H)3 domain. Furthermore the majority of residues were present in all four IgG subclasses. Consequently the peptide identified was consistent with the pan-IgG nature of these antibodies. By using PCImdad, a molecular display programme, this sequence was visualised as surface accessible, located in the C(H)2/C(H)3 inter-domain region and proximal to the residue arginine(435). It is speculated that this residue may be important for phenotypic expression of G3m(u) and specificity of these reagents. Pepscan analysis of MAbs G7C and JD312 (both pan-IgG) highlighted the core peptide sequence 290-KPREE-294, which was present in the C(H)2 domain and was common to all four IgG subclasses. PCImdad also showed this region to be highly accessible and was consistent with previous bioinformatic and autoimmune analysis of IgG. Overall these MAbs may serve as useful anti-IgG or anti-G3m(u) reagents and probes of immunoglobulin structure.en
dc.format.extent399074 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T75-48BM4JT-4&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=5c4e2c50f2e7c01a63119cee94260d4d-
dc.subjectMonoclonal antibodiesen
dc.subjectAssay restrictionen
dc.subjectEpitope mappingen
dc.subjectIgG allotypesen
dc.titleCharacterisation of epitopes of pan-IgG/anti-G3m(u) and anti-Fc monoclonal antibodies.en
dc.typeArticleen
dc.format.digYES-
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