Screening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease.

2.50
Hdl Handle:
http://hdl.handle.net/2436/7750
Title:
Screening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease.
Authors:
Linke, A.T.; Marchant, B.; Marsh, P.; Frampton, Geoffrey; Murphy, John J.; Rose, Marlene L.
Abstract:
A HUVEC cDNA library was screened with sera from two patients who had developed transplant-associated coronary artery disease (TxCAD) following cardiac transplantation. A total of six positive clones were isolated from a primary screen of 40 000 genes. Subsequent DNA sequence analysis identified these to be lysyl tRNA synthetase, ribosomal protein L7, ribosomal protein L9, beta transducin and TANK. Another gene whose product could not be identified showed homology to a human cDNA clone (DKFZp566M063) derived from fetal kidney. Full-length constructs of selected genes were expressed as his-tag recombinant fusion proteins and used to screen a wider patient base by ELISA to determine prevalence and association with TxCAD. Of these ribosomal protein L7 showed the highest prevalence (55.6%) with TxCAD sera compared to 10% non-CAD.
Citation:
Clinical & Experimental Immunology, 126(1): 173-179
Publisher:
Wiley InterScience
Issue Date:
2001
URI:
http://hdl.handle.net/2436/7750
DOI:
10.1046/j.1365-2249.2001.01654.x
PubMed ID:
11678915
Additional Links:
http://www3.interscience.wiley.com/journal/120697614/abstract
Submitted date:
2007-01-24
Type:
Article
Language:
en
ISSN:
0009-9104
Appears in Collections:
Molecular Immunology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorLinke, A.T.-
dc.contributor.authorMarchant, B.-
dc.contributor.authorMarsh, P.-
dc.contributor.authorFrampton, Geoffrey-
dc.contributor.authorMurphy, John J.-
dc.contributor.authorRose, Marlene L.-
dc.date.accessioned2007-01-24T16:36:20Z-
dc.date.available2007-01-24T16:36:20Z-
dc.date.issued2001-
dc.date.submitted2007-01-24-
dc.identifier.citationClinical & Experimental Immunology, 126(1): 173-179en
dc.identifier.issn0009-9104-
dc.identifier.pmid11678915-
dc.identifier.doi10.1046/j.1365-2249.2001.01654.x-
dc.identifier.urihttp://hdl.handle.net/2436/7750-
dc.description.abstractA HUVEC cDNA library was screened with sera from two patients who had developed transplant-associated coronary artery disease (TxCAD) following cardiac transplantation. A total of six positive clones were isolated from a primary screen of 40 000 genes. Subsequent DNA sequence analysis identified these to be lysyl tRNA synthetase, ribosomal protein L7, ribosomal protein L9, beta transducin and TANK. Another gene whose product could not be identified showed homology to a human cDNA clone (DKFZp566M063) derived from fetal kidney. Full-length constructs of selected genes were expressed as his-tag recombinant fusion proteins and used to screen a wider patient base by ELISA to determine prevalence and association with TxCAD. Of these ribosomal protein L7 showed the highest prevalence (55.6%) with TxCAD sera compared to 10% non-CAD.en
dc.format.extent99677 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherWiley InterScienceen
dc.relation.urlhttp://www3.interscience.wiley.com/journal/120697614/abstracten
dc.subjectCoronary Artery Diseaseen
dc.subjectHUVEC cDNAen
dc.subjectRPL7en
dc.titleScreening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease.en
dc.typeArticleen
dc.format.digYES-

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