University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Pharmacology Research Group > Structural and functional characterization of the human CD36 gene promoter: identification of a proximal PEBP2/CBF site.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/7742
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: Structural and functional characterization of the human CD36 gene promoter: identification of a proximal PEBP2/CBF site.
Authors: Armesilla, Angel Luis
Calvo, Dominica
Vega, Miguel A.
Citation: The Journal of Biological Chemistry, 271(13): 7781-7787
Publisher: American Society for Biochemistry and Molecular Biology
Issue Date: 1996
URI: http://hdl.handle.net/2436/7742
PubMed ID: 8631821
Additional Links: http://www.jbc.org/cgi/reprint/271/13/7781
Abstract: CD36 is a cell surface glycoprotein composed of a single polypeptide chain, which interacts with thrombospondin, collagens type I and IV, oxidized low density lipoprotein, fatty acids, anionic phospholipids, and erythrocytes parasitized with Plasmodium falciparum. Its expression is restricted to a few cell types, including monocyte/macrophages. In these cells, CD36 is involved in phagocytosis of apoptotic cells, and foam cell formation by uptake of oxidized low density lipoprotein. To study the molecular mechanisms that control the transcription of the CD36 gene in monocytic cells we have isolated and analyzed the CD36 promoter. Transient expression experiments of 5'-deletion fragments of the CD36 promoter coupled to luciferase demonstrated that as few as 158 base pairs upstream from the transcription initiation site were sufficient to direct the monocyte-specific transcription of the reporter gene. Within the above region, the fragment spanning nucleotides -158 to -90 was required for optimal transcription in monocytic cells. Biochemical analysis of the region -158/-90 revealed a binding site for transcription factors of the polyomavirus enhancer-binding protein 2/core-binding factor (PEBP2/CBF) family at position -103. Disruption of the PEBP2/CBF site markedly diminished the role of the PEBP2/CBF factors in the constitutive transcription of the CD36 gene. The involvement of members of the PEBP2/CBF family in chromosome translocations associated with acute myeloid leukemia, and in the transcriptional regulation of the myeloid-specific genes encoding for myeloperoxidase, elastase, and the colony-stimulating factor receptor, highlights the relevance of the regulation of the CD36 gene promoter in monocytic cells by members of the PEBP2/CBF family.
Type: Article
Language: en
Keywords: CD36 Gene Promoter
Glycoproteins
PEBP2/CBF
ISSN: 0021-9258
Appears in Collections: Molecular Pharmacology Research Group

Files in This Item:
File Description Size Format View/Open
Armesilla7.pdf753KbAdobe PDFThumbnail
View/Open

Related articles on PubMed
bullet
bullet
bullet
bullet
bullet
Subcellular localization of the alpha and beta subunits of the acute myeloid leukemia-linked transcription factor PEBP2/CBF.
Lu J, Maruyama M, Satake M, Bae SC, Ogawa E, Kagoshima H, Shigesada K, Ito Y
1995 Mar
See all 181 articles

All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies