2.50
Hdl Handle:
http://hdl.handle.net/2436/7692
Title:
Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility.
Authors:
Schuh, Kai; Cartwright, Elizabeth J.; Jankevics, Eriks; Bundschu, Karin; Liebermann, Jürgen; Williams, Judith C.; Armesilla, Angel Luis; Emerson, Michael; Oceandy, Delvac; Knobeloch, Klaus-Peter; Neyses, Ludwig
Abstract:
Calcium and Ca(2+)-dependent signals play a crucial role in sperm motility and mammalian fertilization, but the molecules and mechanisms underlying these Ca(2+)-dependent pathways are incompletely understood. Here we show that homozygous male mice with a targeted gene deletion of isoform 4 of the plasma membrane calcium/calmodulin-dependent calcium ATPase (PMCA), which is highly enriched in the sperm tail, are infertile due to severely impaired sperm motility. Furthermore, the PMCA inhibitor 5-(and-6)-carboxyeosin diacetate succinimidyl ester reduced sperm motility in wild-type animals, thus mimicking the effects of PMCA4 deficiency on sperm motility and supporting the hypothesis of a pivotal role of the PMCA4 on the regulation of sperm function and intracellular Ca(2+) levels.
Citation:
The Journal of Biological Chemistry, 279(27): 28220-28226
Publisher:
American Society for Biochemistry and Molecular Biology
Issue Date:
2004
URI:
http://hdl.handle.net/2436/7692
DOI:
10.1074/jbc.M312599200
PubMed ID:
15078889
Additional Links:
http://www.jbc.org/cgi/content/abstract/279/27/28220; http://direct.bl.uk/bld/PlaceOrder.do?UIN=152341633&ETOC=RN&from=searchengine
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
Molecular Pharmacology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSchuh, Kai-
dc.contributor.authorCartwright, Elizabeth J.-
dc.contributor.authorJankevics, Eriks-
dc.contributor.authorBundschu, Karin-
dc.contributor.authorLiebermann, Jürgen-
dc.contributor.authorWilliams, Judith C.-
dc.contributor.authorArmesilla, Angel Luis-
dc.contributor.authorEmerson, Michael-
dc.contributor.authorOceandy, Delvac-
dc.contributor.authorKnobeloch, Klaus-Peter-
dc.contributor.authorNeyses, Ludwig-
dc.date.accessioned2007-01-23T15:08:58Z-
dc.date.available2007-01-23T15:08:58Z-
dc.date.issued2004-
dc.identifier.citationThe Journal of Biological Chemistry, 279(27): 28220-28226en
dc.identifier.issn0021-9258-
dc.identifier.pmid15078889-
dc.identifier.doi10.1074/jbc.M312599200-
dc.identifier.urihttp://hdl.handle.net/2436/7692-
dc.description.abstractCalcium and Ca(2+)-dependent signals play a crucial role in sperm motility and mammalian fertilization, but the molecules and mechanisms underlying these Ca(2+)-dependent pathways are incompletely understood. Here we show that homozygous male mice with a targeted gene deletion of isoform 4 of the plasma membrane calcium/calmodulin-dependent calcium ATPase (PMCA), which is highly enriched in the sperm tail, are infertile due to severely impaired sperm motility. Furthermore, the PMCA inhibitor 5-(and-6)-carboxyeosin diacetate succinimidyl ester reduced sperm motility in wild-type animals, thus mimicking the effects of PMCA4 deficiency on sperm motility and supporting the hypothesis of a pivotal role of the PMCA4 on the regulation of sperm function and intracellular Ca(2+) levels.en
dc.format.extent529970 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen
dc.relation.urlhttp://www.jbc.org/cgi/content/abstract/279/27/28220en
dc.relation.urlhttp://direct.bl.uk/bld/PlaceOrder.do?UIN=152341633&ETOC=RN&from=searchengine-
dc.subjectPlasma Membraneen
dc.subjectSperm Motilityen
dc.subjectMale Fertilityen
dc.subjectCa2+en
dc.titlePlasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility.en
dc.typeArticleen
dc.format.digYES-

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