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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Diabetes, Physiology and Molecular Medicine Research Group > Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/6313
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Title: Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets.
Authors: Brown, James E. P.
Thomas, Steven
Digby, Janet E.
Dunmore, Simon J.
Citation: FEBS Letters, 513(2-3): 189-192
Publisher: Elsevier BV
Issue Date: 2002
URI: http://hdl.handle.net/2436/6313
DOI: 10.1016/S0014-5793(02)02296-2
PubMed ID: 11904148
Additional Links: http://www.ingentaconnect.com/content/els/00145793/2002/00000513/00000002/art02296
Abstract: Elevated islet uncoupling protein-2 (UCP-2) impairs beta-cell function and UCP-2 may be increased in clinical obesity and diabetes. We investigated the effects of glucose and leptin on UCP-2 expression in isolated human islets. Human islets were incubated for 24 h with glucose (5.5-22 mmol/l)+/-leptin (0-10 nmol/l). Some islet batches were incubated at high (22 mmol/l), and subsequently lower (5.5 mmol/l), glucose to assess reversibility of effects. Leptin effects on insulin release were also measured. Glucose dose-dependently increased UCP-2 expression in all islet batches, maximally by three-fold. This was not fully reversed by subsequently reduced glucose levels. Leptin decreased UCP-2 expression by up to 75%, and maximally inhibited insulin release by 47%, at 22 mmol/l glucose. This is the first report of UCP-2 expression in human islets and provides novel evidence of its role in the loss of beta-cell function in diabetes.
Type: Article
Language: en
Keywords: Gene Expression
Glucose toxicity
Type 2 diabetes
β-Cell
ISSN: 0014-5793
Appears in Collections: Diabetes, Physiology and Molecular Medicine Research Group

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