2.50
Hdl Handle:
http://hdl.handle.net/2436/6313
Title:
Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets.
Authors:
Brown, James E. P.; Thomas, Steven; Digby, Janet E.; Dunmore, Simon J.
Abstract:
Elevated islet uncoupling protein-2 (UCP-2) impairs beta-cell function and UCP-2 may be increased in clinical obesity and diabetes. We investigated the effects of glucose and leptin on UCP-2 expression in isolated human islets. Human islets were incubated for 24 h with glucose (5.5-22 mmol/l)+/-leptin (0-10 nmol/l). Some islet batches were incubated at high (22 mmol/l), and subsequently lower (5.5 mmol/l), glucose to assess reversibility of effects. Leptin effects on insulin release were also measured. Glucose dose-dependently increased UCP-2 expression in all islet batches, maximally by three-fold. This was not fully reversed by subsequently reduced glucose levels. Leptin decreased UCP-2 expression by up to 75%, and maximally inhibited insulin release by 47%, at 22 mmol/l glucose. This is the first report of UCP-2 expression in human islets and provides novel evidence of its role in the loss of beta-cell function in diabetes.
Citation:
FEBS Letters, 513(2-3): 189-192
Publisher:
Elsevier BV
Issue Date:
2002
URI:
http://hdl.handle.net/2436/6313
DOI:
10.1016/S0014-5793(02)02296-2
PubMed ID:
11904148
Additional Links:
http://www.ingentaconnect.com/content/els/00145793/2002/00000513/00000002/art02296
Submitted date:
2006-11-30
Type:
Article
Language:
en
ISSN:
0014-5793
Appears in Collections:
Diabetes, Physiology and Molecular Medicine Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorBrown, James E. P.-
dc.contributor.authorThomas, Steven-
dc.contributor.authorDigby, Janet E.-
dc.contributor.authorDunmore, Simon J.-
dc.date.accessioned2006-11-30T16:40:19Z-
dc.date.available2006-11-30T16:40:19Z-
dc.date.issued2002-
dc.date.submitted2006-11-30-
dc.identifier.citationFEBS Letters, 513(2-3): 189-192en
dc.identifier.issn0014-5793-
dc.identifier.pmid11904148-
dc.identifier.doi10.1016/S0014-5793(02)02296-2-
dc.identifier.urihttp://hdl.handle.net/2436/6313-
dc.description.abstractElevated islet uncoupling protein-2 (UCP-2) impairs beta-cell function and UCP-2 may be increased in clinical obesity and diabetes. We investigated the effects of glucose and leptin on UCP-2 expression in isolated human islets. Human islets were incubated for 24 h with glucose (5.5-22 mmol/l)+/-leptin (0-10 nmol/l). Some islet batches were incubated at high (22 mmol/l), and subsequently lower (5.5 mmol/l), glucose to assess reversibility of effects. Leptin effects on insulin release were also measured. Glucose dose-dependently increased UCP-2 expression in all islet batches, maximally by three-fold. This was not fully reversed by subsequently reduced glucose levels. Leptin decreased UCP-2 expression by up to 75%, and maximally inhibited insulin release by 47%, at 22 mmol/l glucose. This is the first report of UCP-2 expression in human islets and provides novel evidence of its role in the loss of beta-cell function in diabetes.en
dc.format.extent160852 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://www.ingentaconnect.com/content/els/00145793/2002/00000513/00000002/art02296en
dc.subjectGene Expressionen
dc.subjectGlucose toxicityen
dc.subjectType 2 diabetesen
dc.subjectβ-Cellen
dc.titleGlucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets.en
dc.typeArticleen
dc.format.digYES-

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